Overview

  • Product nameAnti-L3MBTL3 antibody
    See all L3MBTL3 primary antibodies
  • Description
    Mouse polyclonal to L3MBTL3
  • Tested applicationsIP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    L3MBTL3 (AAH60845.1, 1 a.a. ~ 755 a.a) full-length human protein.

  • Positive control
    • HeLa cells, transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 88 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • RelevancePutative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Required for normal maturation of myeloid progenitor cells.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • L3MBTL3 antibody
    • Hl(3)mbt like protein antibody
    • KIAA1798 antibody
    • l(3)mbt like 3 (Drosophila) antibody
    • l(3)mbt like 3 antibody
    • L(3)mbt like 3 protein antibody
    • Lethal(3)malignant brain tumor like 3 protein antibody
    • MBT1 antibody
    • RP11-73O6.1 antibody
    see all

Anti-L3MBTL3 antibody images

  • All lanes : Anti-L3MBTL3 antibody (ab68117) at 1/500 dilution

    Lane 1 : L3MBTL3 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

    Predicted band size : 88 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of HeLa cells using purified ab68117 at 10 µg/ml followed by fluorescein conjugated secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% Triton X-100.
  • ab68117 used in Immunoprecipitation.
    Whole cell lysate from cells overexpressing L3MBTL3 tagged with GFP was loaded at 250000µg.
    Protein G was used in the Immunoprecipitation step.
    ab68117 used at 20µl/mg lysate.
    The western blot antibody was a rat anti-GFP polyclonal conjugated to agarose, used at a 1/5000 dilution.

    See Abreview

References for Anti-L3MBTL3 antibody (ab68117)

ab68117 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Hela Cells)
Total protein in input 250000 µg
Specification Hela Cells
Immuno-precipitation step Protein G
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Abcam user community

Verified customer

Submitted Nov 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela Cells)
Loading amount 30 µg
Specification Hela Cells
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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Verified customer

Submitted Oct 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"