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Synthetic peptide, corresponding to a region within amino acids 550-600 of Human L3MBTL3 (NP_115814.1)
Our Abpromise guarantee covers the use of ab99928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at 2-5 µg/mg of lysate.|
Immunoprecipitation analysis of HeLa whole cell lysate (1mg/IP; 20% of IP loaded/lane) using ab99928 at 3µg/mg lysate. A rabbit anti-human L3MBTL3 was used for the western blot at 1µg/ml.
Left Lane - ab99928 IP, Right Lane - control IgG.
ab99928 has not yet been referenced specifically in any publications.