Overview

  • Product name
    Anti-LacI antibody [9A5]
  • Description
    Mouse monoclonal [9A5] to LacI
  • Specificity
    The clone number has been updated from (0.T.81) to (9A5) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Recombinant TrpE fusion protein containing full length E. coli LacI.

  • Positive control
    • Lac1 transfected Cos-1 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 30% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    9A5
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IHC-FoFr
  • Application notes
    ICC/IF: Use at an assay dependent dilution.
    IHC-Fo: Use at an assay dependent dilution. The antibody shows nuclear immunostaining in Lac1 transgenic tissue fixed with 4% paraformaldehyde and permeabilized with Triton X100.
    WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Cellular localization
      Nuclear
    • Database links
      • Alternative names
        • DNA binding transcriptional repressor antibody
        • LacI antibody
        • Transcriptional repressor of the lac operon antibody

      Images

      • This image is courtesy of an anonymous Abreview

        Ab33832 (1/1000) staining Lacl in Escherichia coli cultured cell lysate
        Secondary Antibody: An HRP-conjugated Donkey anti-mouse monoclonal (1/20000)

        Lane 1: Molecular weight Marker
        Lane 2: Rainbow Molecular weight Marker
        Lane 3: Blank
        Lane 4: pCMV Vector cell lysate 1
        Lane 5: pCMV Vector cell lysate 2

        Performed under reduced and denaturing running conditions.
        Blocking step: 5% Milk for 1 hour at 22°C.

        See Abreview

      References

      This product has been referenced in:
      • Russell CW & Mulvey MA The Extraintestinal Pathogenic Escherichia coli Factor RqlI Constrains the Genotoxic Effects of the RecQ-Like Helicase RqlH. PLoS Pathog 11:e1005317 (2015). Read more (PubMed: 26636713) »
      • Sienski G  et al. Silencio/CG9754 connects the Piwi-piRNA complex to the cellular heterochromatin machinery. Genes Dev 29:2258-71 (2015). Human . Read more (PubMed: 26494711) »

      See all 4 Publications for this product

      Customer reviews and Q&As

      Thank you for contacting us. I am sorry to hear that the antibody does not seem to work as exptected. As we discussed, loading more protein (20-30 ug) might help to solve the problem. Should thisnot help, please let me know so that we can discuss other...

      Read More
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Western blot
      Sample
      Escherichia coli Cell lysate - whole cell (Cultured cell lysate)
      Loading amount
      40 µg
      Specification
      Cultured cell lysate
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Username

      Abcam user community

      Verified customer

      Submitted Nov 27 2009

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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