The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 37 kDa.
Use a concentration of 10 µg/ml.
Use a concentration of 5 µg/ml.
Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
PathwayFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Involvement in diseaseDefects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
Sequence similaritiesBelongs to the LDH/MDH superfamily. LDH family.
Overlay histogram showing HeLa cells stained with ab55433 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55433, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Lactate Dehydrogenase antibody (ab55433) used in immunofluorescence at 10ug/ml on HeLa cells.
IHC-P - Lactate Dehydrogenase antibody (ab55433)
Lactate Dehydrogenase antibody (ab55433) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human tonsil tissue.
Western blot - Lactate Dehydrogenase antibody (ab55433)
Predicted band size : 37 kDa Lactate Dehydrogenase antibody (ab55433) at 1ug/lane + HeLa cell lysate at 25ug/lane.
References for Anti-Lactate Dehydrogenase antibody (ab55433)
This product has been referenced in:
Maxwell GL et al. Proteomic analysis of stage I endometrial cancer tissue: Identification of proteins associated with oxidative processes and inflammation. Gynecol Oncol : (2011).
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