Anti-Lactate Dehydrogenase antibody [AF14A11] (ab85326)

Overview

  • Product name
    Anti-Lactate Dehydrogenase antibody [AF14A11]
    See all Lactate Dehydrogenase primary antibodies
  • Description
    Mouse monoclonal [AF14A11] to Lactate Dehydrogenase
  • Tested applications
    Suitable for: Flow Cyt, ChIP, WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    His tagged recombinant Human Lactate Dehydrogenase protein purified from E.coli.

  • Positive control
    • HeLa, MCF7, HepG2, 293T, SK-N-MC, RD and IMR32 cell lysates.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR181608 will be from tissue culture supernatant.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab85326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ChIP Use at an assay dependent concentration.
WB Use a concentration of 0.1 µg/ml. Detects a band of approximately 30 kDa.
IP Use at an assay dependent concentration.

Use at 2μg.

ELISA Use at an assay dependent concentration.

Target

  • Pathway
    Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
  • Involvement in disease
    Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
  • Sequence similarities
    Belongs to the LDH/MDH superfamily. LDH family.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation-inducing gene 19 protein antibody
    • GSD11 antibody
    • L lactate dehydrogenase B chain antibody
    • L-lactate dehydrogenase A chain antibody
    • Lactate dehydrogenase A antibody
    • Lactate dehydrogenase B antibody
    • Lactate dehydrogenase H chain antibody
    • Lactate dehydrogenase M antibody
    • LDH A antibody
    • LDH B antibody
    • LDH H antibody
    • LDH heart subunit antibody
    • LDH M antibody
    • LDH muscle subunit antibody
    • LDH-A antibody
    • LDH-M antibody
    • LDH1 antibody
    • ldha antibody
    • LDHA_HUMAN antibody
    • LDHBD antibody
    • LDHM antibody
    • MS1111 antibody
    • PIG19 antibody
    • Proliferation inducing gene 19 antibody
    • Renal carcinoma antigen NY REN 46 antibody
    • Renal carcinoma antigen NY-REN-59 antibody
    • TRG 5 antibody
    • TRG5 antibody
    see all

Images

  • All lanes : Anti-Lactate Dehydrogenase antibody [AF14A11] (ab85326) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : 293T cell lysate
    Lane 5 : SK-N-MC cell lysate
    Lane 6 : RD cell lysate
    Lane 7 : IMR32 cell lysate


    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HeLa cells stained with ab85326 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85326, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:
  • Tseng CY & Firestein BL The Role of PSD-95 and Cypin in Morphological Changes in Dendrites Following Sublethal NMDA Exposure. J Neurosci 31:15468-80 (2011). WB ; Rat . Read more (PubMed: 22031893) »

See 1 Publication for this product

Customer reviews and Q&As

ab85326 was raised against recombinant human lactate dehydrogenase, not a synthetic peptide, and the antibody has not been epitope mapped.
The antibody is specific to LDHA, which is found predominantly in muscle tissue.
We have tested the cross...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116)
Loading amount
10 µg
Specification
HCT116
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

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Verified customer

Submitted Oct 13 2010

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