Immunocytochemistry/ Immunofluorescence - Lactate Dehydrogenase B antibody [10E6AA9] (ab112996)
ab112996 at 2ug/ml staining Lactate Dehydrogenase B in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). The target protein locates mainly to the cytoplasm.
Flow Cytometry - Lactate Dehydrogenase B antibody [10E6AA9] (ab112996)
ab112996 at 1ug/ml staining Lactate Dehydrogenase B in Triton X-100 permeabilized HeLa cells by flow cytometry (blue). Isotype control antibody (red).
Immunoprecipitation - Anti-Lactate Dehydrogenase B antibody [10E6AA9] (ab112996)
Isolation of LDHB using ab112996 by immunoprecipitation.
The identity of the protein was confirmed by mass spectrometry. The gel was stained with colloidal Coomassie blue G.