Anti-Lactate Dehydrogenase B antibody [10E6AA9] (ab112996)


  • Product name
    Anti-Lactate Dehydrogenase B antibody [10E6AA9]
    See all Lactate Dehydrogenase B primary antibodies
  • Description
    Mouse monoclonal [10E6AA9] to Lactate Dehydrogenase B
  • Specificity
    Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.
  • Tested applications
    Suitable for: ICC/IF, IP, In-Cell ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Hela cell cytosol fraction

  • Positive control
    • HeLa cells and cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.



Our Abpromise guarantee covers the use of ab112996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 2 µg/ml.
IP Use at an assay dependent concentration.
In-Cell ELISA Use a concentration of 2 µg/ml.
Flow Cyt Use a concentration of 1 - 2 µg/ml. Triton permeabilization required.

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • Pathway
    Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
  • Involvement in disease
    Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease.
  • Sequence similarities
    Belongs to the LDH/MDH superfamily. LDH family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis secretory protein Li 281 antibody
    • HEL S 281 antibody
    • L lactate dehydrogenase B chain antibody
    • L-lactate dehydrogenase B chain antibody
    • Lactate dehydrogenase H chain antibody
    • LDH B antibody
    • LDH H antibody
    • LDH heart subunit antibody
    • LDH-B antibody
    • LDH-H antibody
    • LDHB antibody
    • LDHB_HUMAN antibody
    • LDHBD antibody
    • LDHH antibody
    • Renal carcinoma antigen NY REN 46 antibody
    • Renal carcinoma antigen NY-REN-46 antibody
    • TRG-5 antibody
    • TRG5 antibody
    see all

Anti-Lactate Dehydrogenase B antibody [10E6AA9] images

  • ab112996 at 2ug/ml staining Lactate Dehydrogenase B in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). The target protein locates mainly to the cytoplasm.
  • ab112996 at 1ug/ml staining Lactate Dehydrogenase B in Triton X-100 permeabilized HeLa cells by flow cytometry (blue). Isotype control antibody (red).
  • Isolation of LDHB using ab112996 by immunoprecipitation.
    The identity of the protein was confirmed by mass spectrometry. The gel was stained with colloidal Coomassie blue G.

References for Anti-Lactate Dehydrogenase B antibody [10E6AA9] (ab112996)

ab112996 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab112996.
Please use the links above to contact us or submit feedback about this product.


Sign up