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His tagged recombinant Human lactate dehydrogenase B protein, purified from E.coli.
Our Abpromise guarantee covers the use of ab85319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|Flow Cyt||Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|WB||1/5000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).|
|IP||Use at an assay dependent concentration. Use 5µl.|
|ELISA||1/5000 - 1/10000.|
|IHC-P||Use a concentration of 5 µg/ml.|