The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/16000.
IHC: Use at an assay dependent dilution. Strong signal in seminiferous tubules of Mouse Testis.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPossible role in sperm motility.
PathwayFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Sequence similaritiesBelongs to the LDH/MDH superfamily. LDH family.