Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab85472)

Overview

  • Product nameAnti-Lactate Dehydrogenase Isoenzyme V antibody
    See all Lactate Dehydrogenase Isoenzyme V primary antibodies
  • Description
    Rabbit polyclonal to Lactate Dehydrogenase Isoenzyme V
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Lactate Dehydrogenase Isoenzyme V.

    (Peptide available as ab95036.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: HeLa Whole Cell; Human Kidney Tissue; HEK293 Whole Cell; Human Liver Tissue; HepG2 Whole Cell; Human Cervix Tissue; Mouse Kidney Tissue; Mouse Liver Tissue; Rat Liver Tissue; Rat Heart Tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab85472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).Can be blocked with Rabbit polyclonal to ERGI3 - Aminoterminal end (ab95306).

Target

  • RelevanceLactate dehydrogenase 5 (LDH5) is one of five LDH isoenzymes and important for promoting anaerobic glycolysis. LDH5 is transcriptionally regulated by the hypoxia inducible factors (HIF) 1a and 2a.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • Cell proliferation-inducing gene 19 protein antibody
    • L lactate dehydrogenase A chain antibody
    • Lactate dehydrogenase A antibody
    • LDH A antibody
    • LDH M antibody
    • LDH muscle subunit antibody
    • LDH1 antibody
    • LDH5 antibody
    • LDHA antibody
    • PIG 19 antibody
    • PIG19 antibody
    • Renal carcinoma antigen NY-REN-59 antibody
    see all

Anti-Lactate Dehydrogenase Isoenzyme V antibody images

  • All lanes : Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab85472) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : Human liver tissue lysate - total protein (ab29889)
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : Human cervix normal tissue lysate - total protein (ab29226)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 74 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab85472 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85472, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab85472) at 1 µg/ml + Natural human Lactate Dehydrogenase Isoenzyme V protein (ab77945) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 12 minutes
  • All lanes : Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab85472) at 1 µg/ml

    Lane 1 : Kidney (Mouse) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 170 kDa,49 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds

References for Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab85472)

ab85472 has not yet been referenced specifically in any publications.

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