Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab9002)

Abreviews (3)Q&A (4)Specific References (12)
Customer reviews and FAQs

Overview

  • Product nameAnti-Lactate Dehydrogenase Isoenzyme V antibody
    See all Lactate Dehydrogenase Isoenzyme V primary antibodies
  • Description
    Sheep polyclonal to Lactate Dehydrogenase Isoenzyme V
  • SpecificityIdentity has been confirmed by double diffusion (Ouchterlony) against purified LDH V and a known anti-human LDH V. Specificity has been demonstrated by Western blot against Liver Cell Lysate. We have never performed a cross-reactivity check to LDH I, II, III, or IV, however, due to the similarity between LDH V and LDH's II, III and IV there is a possibility that there will be some cross-reactivity.
  • Tested applicationsSuitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human Lactate Dehydrogenase V, purified from human placenta.

  • Positive control
    • This antibody gave a positive signal in the following lysates: Human Kidney Tissue; Human Liver Tissue; HEK293 Whole Cell; HepG2 Whole Cell.

Properties

Applications

Our Abpromise guarantee covers the use of ab9002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution. PubMed: 12942121
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IHC-Fr 1/100. PubMed: 21569415Snap freeze tissue in liquid nitrogen and store. Then the sections should be fixed for 10 minutes in acetone and rehydrated in PBS.

Target

  • RelevanceLactate dehydrogenase 5 (LDH5) is one of five LDH isoenzymes and important for promoting anaerobic glycolysis. LDH5 is transcriptionally regulated by the hypoxia inducible factors (HIF) 1a and 2a.
  • Cellular localizationCytoplasmic
  • Database links
    • Alternative names
      • Cell proliferation-inducing gene 19 protein antibody
      • L lactate dehydrogenase A chain antibody
      • Lactate dehydrogenase A antibody
      • LDH A antibody
      • LDH M antibody
      • LDH muscle subunit antibody
      • LDH1 antibody
      • LDH5 antibody
      • LDHA antibody
      • PIG 19 antibody
      • PIG19 antibody
      • Renal carcinoma antigen NY-REN-59 antibody
      see all

    Anti-Lactate Dehydrogenase Isoenzyme V antibody images

    • Western blot - Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab9002)
      Western blot - Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab9002)
      All lanes : Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab9002) at 1 µg/ml

      Lane 1 : Human kidney tissue lysate - total protein (ab30203)
      Lane 2 : Human liver tissue lysate - total protein (ab29889)
      Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (HRP) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 36 kDa
      Observed band size : 36 kDa


      Exposure time : 30 seconds
    • Immunohistochemistry (Frozen sections) - Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab9002)
      Immunohistochemistry (Frozen sections) - Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab9002)Image from Rademakers SE et al., BMC Cancer. 2011 May 12;11:167. Fig 2.; doi:10.1186/1471-2407-11-167; 12 May 2011, BMC Cancer 2011, 11:167
      Immunohistochemical analysis of Human head and neck cancer tissue, staining Lactate Dehydrogenase Isoenzyme V (red) with ab9002 at 1/100 dilution. Sections were fixed in acetone before being incubated with primary antibody overnight at 4°C. A Cy3-conjugated donkey anti-sheep IgG (1/600) was used as the secondary antibody.

    References for Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab9002)

    This product has been referenced in:

    See all 12 Publications for this product

    Publishing research using ab9002? Please let us know so that we can cite the reference in this datasheet.

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    Submit an abreview for ab9002 Submit a question for ab9002

    7 Abreviews or Q&A

    10
    Votes: Highest First

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Lactate Dehydrogenase Isoenzyme V antibody

     Excellent
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Bladder Tumor Cell Line)
    Permeabilization Yes - 01% v/v Triton X-100
    Specification Bladder Tumor Cell Line
    Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative Paraformaldehyde
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    Username

    Dr. Dimitra Kalamida

    Verified customer

    Submitted Sep 12 2016

    Western blot abreview for Anti-Lactate Dehydrogenase Isoenzyme V antibody

     Good
    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (prostate cancer cell line)
    Gel Running Conditions Reduced Denaturing (10%acrylamide)
    Loading amount 20 µg
    Specification prostate cancer cell line
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Username

    Dr. Stamatia Pouliliou

    Verified customer

    Submitted Jun 26 2015

    Western blot abreview for Anti-Lactate Dehydrogenase Isoenzyme V antibody

     Excellent
    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (Epithelial cells)
    Gel Running Conditions Non-reduced Denaturing (10)
    Loading amount 30 µg
    Specification Epithelial cells
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Username

    Mr. Achilleas Mitrakas

    Verified customer

    Submitted Jun 15 2015

    Phone call requesting:
    1. positive control tissue to use

    2. what AR to use (the use microwave, will pressure cooker be ok) and which buffer
    3. will novalink system work with the ab?

    Thank you for your phone call and your interest in our products.
    As discussed over the phone, the Anti-Lactate Dehydrogenase Isoenzyme V antibody (ab9002) has been used by several research groups in performing immunohistochemical staining. Having r...

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    sequence homology with mouse is 94%
    will try in mouse

    Thank you for your phone call.

    DISCOUNT CODE: xxx
    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test ab9002 in mouse. This code will give you: 1 free PRIMARY ANTIBODY before the expirati...

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    I am looking into ordering a LDH 5 antibody and there are two possibilities I am interested in ab47010 and ab9002. The ab9002 states as part of the alternative name list that it recognises LDH5 but the ab47010 does not. I am interested in the ab47010 ...

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    Thank you for contacting Abcam. I’ve had a look into this and done a bit of a web search and as far as I can see LDH5, LDHM and LDHA are all different names for the same protein, as stated in this paper, http://www.pnas.org/content/107/5/2037.long....

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    Customer would like to know what size band is expected using ab9002.

    From our testing we would expect our anti LDH-V to show a single band of approximately 36 kDa by Western blot - this band corresponds to the A subunit (sometimes called the muscle subunit) which LDH-V is entirely composed of.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"