Human Predicted to work with:
Mouse, Rabbit, Cat, Dog, Chimpanzee, Zebrafish, Spider monkey
Synthetic peptide: PLSCHGWVLG EHGDSSVPVW SGMNVAGVSL KTLHPDLGTD KDKEQWKEVH , corresponding to a region within internal sequence amino acids 182 - 231 of Human Lactate Dehydrogenase isozyme H4 (NP_005557)
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Lactate dehydrogenase is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. Isoforms of the enzyme include LDH 1 (4H) in the heart, LDH 2 (3H1M) in the reticuloendothelial system, LDH 3 (2H2M) in the lungs, LDH 4 (1H3M) in the kidneys and LDH 5 (4M) in the liver and striated muscle. The M and H subunits are encoded by two different genes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling Lactate Dehydrogenase isozyme H4 with ab81485 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Lactate Dehydrogenase isozyme H4. Right - Merge.
Western blot - Anti-Lactate Dehydrogenase isozyme H4 antibody (ab81485)
Predicted band size : 37 kDa Western blot analysis of HeLa cell lysate labeling Lactate Dehydrogenase isozyme H4 with ab81485 at 1.0µg/ml.
Western blot - Lactate Dehydrogenase isozyme H4 antibody (ab81485)
Anti-Lactate Dehydrogenase isozyme H4 antibody (ab81485) at 1 µg/ml + 293T cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 37 kDa Observed band size : 37 kDa