Anti-Lactoferrin antibody (ab112968)

Overview

  • Product name
  • Description
    Goat polyclonal to Lactoferrin
  • Tested applications
    Suitable for: IHC-P, WB, ELISA, ICCmore details
  • Species reactivity
    Reacts with: Cow
  • Immunogen

    Immunogen is purified lactoferrin of bovine milk origin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was solid phase adsorbed to ensure specificity. ab112968 was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/2000.
WB 1/1000 - 1/30000. Predicted molecular weight: 78 kDa. Colorimetric: Use 1/1000-1/10000 dilution; Chemiluminescent: Use 1/1000-1/30000 dilution.
ELISA 1/100 - 1/30000. Coating: Use 1/100-1/500 dilution; Primary: Use 1/1000-1/30000 dilution.
ICC 1/200 - 1/2000.

Target

  • Function
    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
    Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
    Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
    The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
  • Sequence similarities
    Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • GIG12 antibody
    • Growth inhibiting protein 12 antibody
    • HLF2 antibody
    • Kaliocin 1 antibody
    • Lactoferrin antibody
    • Lactoferroxin-C antibody
    • Lactotransferrin antibody
    • LF antibody
    • LTF antibody
    • Neutrophil lactoferrin antibody
    • Talalactoferrin antibody
    • TRFL_HUMAN antibody
    see all

Images

  • All lanes : Anti-Lactoferrin antibody (ab112968) at 1/1000 dilution

    Lane 1 : Resuspended cow lactoferrin powder at 2 µg
    Lane 2 : Resuspended cow lactoferrin powder at 1 µg
    Lane 3 : Resuspended cow lactoferrin powder at 0.5 µg
    Lane 4 : Resuspended cow lactoferrin powder at 0.1 µg
    Lane 5 : Resuspended cow lactoferrin powder at 0.05 µg
    Lane 6 : Resuspended cow lactoferrin powder at 0.01 µg

    Secondary
    Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Federico Benetti.

    Blocked with 3% BSA for 1 hour at 20°C.

    Incubated with the primary antibody for 16 hours at 4°C in blocking buffer.

    Membrane - Nitrocellulose.

    See Abreview

References

ab112968 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Cow Purified protein (Resuspended cow lactoferrin powder)
Gel Running Conditions
Reduced Denaturing (4% stacking 10% resolving)
Loading amount
2 µg
Specification
Resuspended cow lactoferrin powder
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Dr. Federico Benetti

Verified customer

Submitted May 02 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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