Anti-Lactoferrin antibody (ab112968)


  • Product nameAnti-Lactoferrin antibody
    See all Lactoferrin primary antibodies
  • Description
    Goat polyclonal to Lactoferrin
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, ICCmore details
  • Species reactivity
    Reacts with: Cow
  • Immunogen

    Immunogen is purified lactoferrin of bovine milk origin


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was solid phase adsorbed to ensure specificity. ab112968 was isolated by affinity chromatography using antigen coupled to agarose beads.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab112968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/2000.
WB 1/1000 - 1/30000. Predicted molecular weight: 78 kDa. Colorimetric: Use 1/1000-1/10000 dilution; Chemiluminescent: Use 1/1000-1/30000 dilution.
ELISA 1/100 - 1/30000. Coating: Use 1/100-1/500 dilution; Primary: Use 1/1000-1/30000 dilution.
ICC 1/200 - 1/2000.


  • FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
    Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
    Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
    The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
  • Sequence similaritiesBelongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • GIG12 antibody
    • Growth inhibiting protein 12 antibody
    • HLF2 antibody
    • Kaliocin 1 antibody
    • Lactoferrin antibody
    • Lactoferroxin-C antibody
    • Lactotransferrin antibody
    • LF antibody
    • LTF antibody
    • Neutrophil lactoferrin antibody
    • Talalactoferrin antibody
    • TRFL_HUMAN antibody
    see all

Anti-Lactoferrin antibody images

  • All lanes : Anti-Lactoferrin antibody (ab112968) at 1/1000 dilution

    Lane 1 : Resuspended cow lactoferrin powder at 2 µg
    Lane 2 : Resuspended cow lactoferrin powder at 1 µg
    Lane 3 : Resuspended cow lactoferrin powder at 0.5 µg
    Lane 4 : Resuspended cow lactoferrin powder at 0.1 µg
    Lane 5 : Resuspended cow lactoferrin powder at 0.05 µg
    Lane 6 : Resuspended cow lactoferrin powder at 0.01 µg

    Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Federico Benetti.

    Blocked with 3% BSA for 1 hour at 20°C.

    Incubated with the primary antibody for 16 hours at 4°C in blocking buffer.

    Membrane - Nitrocellulose.

    See Abreview

References for Anti-Lactoferrin antibody (ab112968)

ab112968 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Cow Purified protein (Resuspended cow lactoferrin powder)
Gel Running Conditions Reduced Denaturing (4% stacking 10% resolving)
Loading amount 2 µg
Specification Resuspended cow lactoferrin powder
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Dr. Federico Benetti

Verified customer

Submitted May 02 2017