Overview

  • Product name
  • Description
    Goat polyclonal to Lactoferrin
  • Tested applications
    Suitable for: ELISA, WB, Sandwich ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide:

    C-ENYKSQQSSDPDP

    , corresponding to internal sequence amino acids 432-444 of Human Lactoferrin (NP_002334.2).

  • Positive control
    • Human Peripheral Blood Mononucleocyte lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab77548 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 85 kDa.
Sandwich ELISA Use a concentration of 5 - 10 µg/ml.
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
    Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
    Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
    The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
  • Sequence similarities
    Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • GIG12 antibody
    • Growth inhibiting protein 12 antibody
    • HLF2 antibody
    • Kaliocin 1 antibody
    • Lactoferrin antibody
    • Lactoferroxin-C antibody
    • Lactotransferrin antibody
    • LF antibody
    • LTF antibody
    • Neutrophil lactoferrin antibody
    • Talalactoferrin antibody
    • TRFL_HUMAN antibody
    see all

Images

  • Anti-Lactoferrin antibody (ab77548) at 0.05 µg/ml + Human Peripheral Blood Mononucleocyte lysate (in RIPA buffer) at 35 µg

    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
  • ab77548 (2µg/ml) staining of paraffin embedded Human Blood Vessel shows strong staining of cytoplasm of neutrophils. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • Sandwich-type ELISA with increasing amount of recombinant LTF captured by a rabbit antibody. ab77548 reporter concentration: 5 ug/ml
  • Anti-Lactoferrin antibody (ab77548) at 0.2 µg/ml + Human Lactoferrin full length protein (ab78526) at 0.01 µg/ml

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 8 minutes

References

ab77548 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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