Overview

  • Product name
  • Description
    Rabbit polyclonal to Lactoferrin
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non human primatesDoes not react with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Lactoferrin.

    (Peptide available as ab87442.)

  • Positive control
    • This antibody gave a positive signal in the following human samples: Plasma Total Protein; Prostate Tissue Lysate; Lung Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 78 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
    Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
    Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
    The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
  • Sequence similarities
    Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • GIG12 antibody
    • Growth inhibiting protein 12 antibody
    • HLF2 antibody
    • Kaliocin 1 antibody
    • Lactoferrin antibody
    • Lactoferroxin-C antibody
    • Lactotransferrin antibody
    • LF antibody
    • LTF antibody
    • Neutrophil lactoferrin antibody
    • Talalactoferrin antibody
    • TRFL_HUMAN antibody
    see all

Images

  • All lanes : Anti-Lactoferrin antibody (ab77780)

    Lane 1 : Human Plasma Total Protein
    Lane 2 : Human Prostate Tissue Lysate
    Lane 3 : Human Lung Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 80 kDa (possible cleavage fragment).
  • ICC/IF image of ab77780 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77780, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Rageh AA  et al. Lactoferrin Expression in Human and Murine Ocular Tissue. Curr Eye Res 41:883-9 (2016). Read more (PubMed: 26431065) »
  • Val S  et al. Proteomic Characterization of Middle Ear Fluid Confirms Neutrophil Extracellular Traps as a Predominant Innate Immune Response in Chronic Otitis Media. PLoS One 11:e0152865 (2016). IF . Read more (PubMed: 27078692) »

See all 2 Publications for this product

Customer reviews and Q&As

Vielen Dank für Ihre Anfrage.

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten, die wir nicht lösen konnten. Wiegewünschthabe ich eine kostenlose Ersatzlieferung ueber den ab77780 Anti-Lactoferrin Antik&oum...

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Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse Iris)
Loading amount
10 µg
Specification
Mouse Iris
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Blocking step
FISH buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Ms. Abrar Rageh

Verified customer

Submitted Jan 13 2012

Thank you for calling Abcam earlier. Please find the testing discount code below, also below is a link to that explains more about our testing discount program. http://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collabora...

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If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know which antibody you would like to test (Ab77780 or Ab38155). I will then send a discount code. This code must be issued before purchasing the antibody...

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Thanks for your call yesterday and for your patience while I have looked through our lactoferrin antibodies. Ab77780 would be a good antibody to try, as it will probably cross-react with mouse, though I can't say that it won't react with transferrin as...

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