Anti-Lactoferrin antibody (ab77780)


  • Product nameAnti-Lactoferrin antibody
    See all Lactoferrin primary antibodies
  • Description
    Rabbit polyclonal to Lactoferrin
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non Human PrimatesDoes not react with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Lactoferrin.

    (Peptide available as ab87442.)

  • Positive control
    • This antibody gave a positive signal in the following human samples: Plasma Total Protein; Prostate Tissue Lysate; Lung Tissue Lysate.



Our Abpromise guarantee covers the use of ab77780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 78 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
    Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
    Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
    The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
  • Sequence similaritiesBelongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • GIG12 antibody
    • Growth inhibiting protein 12 antibody
    • HLF2 antibody
    • Kaliocin 1 antibody
    • Lactoferrin antibody
    • Lactoferroxin-C antibody
    • Lactotransferrin antibody
    • LF antibody
    • LTF antibody
    • Neutrophil lactoferrin antibody
    • Talalactoferrin antibody
    • TRFL_HUMAN antibody
    see all

Anti-Lactoferrin antibody images

  • All lanes : Anti-Lactoferrin antibody (ab77780)

    Lane 1 : Human Plasma Total Protein
    Lane 2 : Human Prostate Tissue Lysate
    Lane 3 : Human Lung Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 80 kDa (possible cleavage fragment).
  • ICC/IF image of ab77780 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77780, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Lactoferrin antibody (ab77780)

ab77780 has not yet been referenced specifically in any publications.

Product Wall

Vielen Dank für Ihre Anfrage.

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten, die wir nicht lösen konnten. Wiegewünschthabe ich eine kostenlose Ersatzlieferung ueber den ab77780 Anti-Lactoferrin Antik&oum...

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Application Western blot
Sample Mouse Tissue lysate - whole (Mouse Iris)
Loading amount 10 µg
Specification Mouse Iris
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Blocking step FISH buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Ms. Abrar Rageh

Verified customer

Submitted Jan 13 2012

Thank you for calling Abcam earlier. Please find the testing discount code below, also below is a link to that explains more about our testing discount program.

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If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know which antibody you would like to test (Ab77780 or Ab38155). I will then send a discount code. This code must be issued before purchasing the antibody...

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Thanks for your call yesterday and for your patience while I have looked through our lactoferrin antibodies. Ab77780 would be a good antibody to try, as it will probably cross-react with mouse, though I can't say that it won't react with transferrin as...

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