Recombinant
RabMAb

Anti-Lactoferrin antibody [EPR4337] (ab109216)

Overview

  • Product name
    Anti-Lactoferrin antibody [EPR4337]
    See all Lactoferrin primary antibodies
  • Description
    Rabbit monoclonal [EPR4337] to Lactoferrin
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Lactoferrin.

  • Positive control
    • WB: PBMC, HeLa, fetal kidney and Human milk lysates ICC/IF: HepG2 cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 78 kDa).
IP 1/10 - 1/100.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
      Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
      Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
      The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
    • Sequence similarities
      Belongs to the transferrin family.
      Contains 2 transferrin-like domains.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • GIG12 antibody
      • Growth inhibiting protein 12 antibody
      • HLF2 antibody
      • Kaliocin 1 antibody
      • Lactoferrin antibody
      • Lactoferroxin-C antibody
      • Lactotransferrin antibody
      • LF antibody
      • LTF antibody
      • Neutrophil lactoferrin antibody
      • Talalactoferrin antibody
      • TRFL_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Lactoferrin antibody [EPR4337] (ab109216) at 1/1000 dilution

      Lane 1 : PBMC lysate
      Lane 2 : Hela lysate
      Lane 3 : Fetal kidney lysate
      Lane 4 : Human milk lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 78 kDa
      Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    • ab109216, at a 1/100 dilution, staining Lactoferrin in HepG2 cells by Immunocytochemistry/ Immunofluorescence.

    References

    ab109216 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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