Lactoferrin (HLF2) Human ELISA Kit (ab108882)

Overview

  • Product nameLactoferrin (HLF2) Human ELISA KitSee all Lactoferrin kits ...
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.6 ng/ml
  • Range
    0.625 ng/ml - 40 ng/ml
  • Recovery

    = 98 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Lactoferrin (HLF2) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Lactoferrin levels in Human cell culture supernatants, serum, urine, saliva, milk, cerebrospinal fluid and plasma.

    A Lactoferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Lactoferrin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Lactoferrin captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
    Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
    Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
    The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
  • Sequence similaritiesBelongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • GIG12
    • Growth inhibiting protein 12
    • HLF2
    • Lactoferrin
    • Lactoferroxin-C
    • Lactotransferrin
    • LF
    • LTF
    • Neutrophil lactoferrin
    • Talalactoferrin
    • TRFL_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Lactoferrin (HLF2) Human ELISA Kit images

  • Lactoferrin measured in biological fluids with background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve Using ab108882.

Protocols

References for Lactoferrin (HLF2) Human ELISA Kit (ab108882)

ab108882 has not yet been referenced specifically in any publications.

Product Wall

Thanks for your enquiry.

I did some calculations and it seems if one starts with 5 ul milk sample and dilutes it in 6 successive 10-fold dilutions, where the final dilution is 50ul of a 100,000-fold dilution, there would be sufficient buffer ...

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Thank you for your interest. We do not have lactoferrin assay kits with longer shelf lives, but the 6 month expiration that is specifiedis very conservative. We are confidentthe kit will be effective, assuming proper storage, within that period.

Thank you for your enquiry.

I can confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products. However, in ...

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Thank you for contacting us.

The cross reactivity with bovine lactoferrin is less than 1%.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you very much for your email.

We have never tested equine samples. We have tested canine, bovine, monkey, mouse, rat, swine, and rabbit samples. See table below.

Species % Cross Reactivity
Canine 1%
Bovine 0.5%
...

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Thank you for contacting us. Unfortunately, the antibodies mentioned are not suitable to detect these (human) proteins in ELISA. However, we have the following antibodies available: 1) Beta-lactoglobulin: Unfortunately, I was unable to find a human ort...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"