Anti-Lactoperoxidase antibody (ab6628)

Overview

  • Product name
    Anti-Lactoperoxidase antibody
    See all Lactoperoxidase primary antibodies
  • Description
    Sheep polyclonal to Lactoperoxidase
  • Tested applications
    Suitable for: WB, IP, ELISA, Dot blotmore details
  • Species reactivity
    Reacts with: Cow
  • Immunogen

    Lactoperoxidase [Bovine Milk]

Properties

Applications

Our Abpromise guarantee covers the use of ab6628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ELISA
Dot blot
  • Application notes
    Dot: Use at an assay dependent concentration.
    ELISA: Use at an assay dependent concentration.
    IP: Use at an assay dependent concentration.
    WB: Use at an assay dependent concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user however a working dilution of 1:18,000 to 1:75,000 is suggested.
  • Target

    • Relevance
      Lactoperoxidase is a natural antibacterial agent in bovine milk, a medium otherwise rich in its ability to support bacterial growth. Milk lactoperoxidase levels vary among species, ranging from little or none in humans to very high concentrations in guinea pigs. Interestingly, lactoperoxidase from milk is identical to the lacrimal and salivary forms of the enzyme. In addition, the enzyme catalyzes the hydrogen peroxide oxidation of iodine and can be employed in labelling proteins with radioiodine.
    • Cellular localization
      Secreted - extracellular space
    • Database links
    • Alternative names
      • LPO antibody
      • Salivary peroxidase antibody
      • SAPX antibody
      • SPO antibody

    References

    ab6628 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Primary airway cell lysate)
    Loading amount
    25 µg
    Specification
    Primary airway cell lysate
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 06 2009

    Thank you for your email. Ab6628 reacts with lactoperoxidase (LPO) isolated from bovine milk. This antibody has only been tested so far for cross-reactivity with cow, and it should suit your needs. If you have any further questions, please contact us a...

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    To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will fo...

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    We have no information about cross reactivity with human LPO

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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