Lactose Assay Kit (ab83384)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Milk, Other biological fluids
Overview
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Product name
Lactose Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Milk, Other biological fluids -
Assay type
Quantitative -
Assay time
1h 00m -
Product overview
In Abcam's Lactose Assay Kit Lactose is hydrolyzed to glucose and galactose. The galactose is subsequently oxidized generating color (OD570nm) and fluorescence (Ex/Em 535/587 nm). Free galactose can be corrected by a background control in the absence of lactase. The Lactose Assay Kit provides a simple, convenient, and sensitive means for direct measurement of lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). Pretreatment of samples is not required. The kit can be used as a high throughput assay.
Visit our FAQs page for tips and troubleshooting. -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K624 Lactose Colorimetric/Fluorometric Assay Kit. K624-100 is the same size as the 100 test size of ab83384.
Lactose (C12H22O11 FW: 342.3) is an important naturally occurred disaccharide, consisting of one galactose and one glucose. Milk contains ~2-8% lactose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer II 1 x 25ml Developer Solution V 1 vial Lactase Enzyme 1 vial Galactose Enzyme Mix 1 vial Lactose Standard 1 x 100µl OxiRed Probe 1 x 200µl -
Research areas
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Relevance
Lactose (C12H22O11 FW: 342.3) is an important naturally occurring disaccharide along with sucrose and maltose. Lactose makes up around 2-8% of milk. It consists of one galactose and one glucose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage. -
Alternative names
- Milk sugar
Images
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab83384 has been referenced in 5 publications.
- Lee S et al. A genetic variant in SLC30A2 causes breast dysfunction during lactation by inducing ER stress, oxidative stress and epithelial barrier defects. Sci Rep 8:3542 (2018). PubMed: 29476070
- Rudolph MC et al. Thyroid hormone responsive protein Spot14 enhances catalysis of fatty acid synthase in lactating mammary epithelium. J Lipid Res 55:1052-65 (2014). PubMed: 24771867
- Gregor MF et al. The role of adipocyte XBP1 in metabolic regulation during lactation. Cell Rep 3:1430-9 (2013). PubMed: 23623498
- Wahlig JL et al. Impact of high-fat diet and obesity on energy balance and fuel utilization during the metabolic challenge of lactation. Obesity (Silver Spring) 20:65-75 (2012). PubMed: 21720435
- Dempsey C et al. Marginal maternal zinc deficiency in lactating mice reduces secretory capacity and alters milk composition. J Nutr 142:655-60 (2012). Mouse . PubMed: 22357740