Overview

  • Product nameAnti-LAD1 antibody
    See all LAD1 primary antibodies
  • Description
    Mouse monoclonal to LAD1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human, Orangutan
  • Immunogen

    Recombinant fragment: SESVKSRGLP CTELFVAPVG VASKRHLFEK ELAGQSRAEP ASSRKENLRL SGVVTSRLNL WISRTQESGD QDPQEAQKAS SATERTQWGQ KSDSSLDAEV , corresponding to amino acids 418-518 of Human LAD1 (NP_005549) with a tag of 26 kDa. Total MW of immunogen is 37 kDa.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 57 kDa). This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ELISA Use at an assay dependent dilution. Detection limit for this antibody is ~0.1ng/ml if used as a capture antibody.

Target

  • FunctionAnchoring filament protein which is a component of the basement membrane zone.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LAD antibody
    • Lad 1 antibody
    • Lad-1 antibody
    • Lad1 antibody
    • LAD1_HUMAN antibody
    • LADA antibody
    • Ladinin 1 antibody
    • Ladinin-1 antibody
    • Linear IgA bullous dermatosis antigen antibody
    • Linear IgA disease antigen antibody
    • Linear IgA disease antigen homolog antibody
    • MGC10355 antibody
    see all

Anti-LAD1 antibody images

  • Anti-LAD1 antibody (ab76610) at 1/500 dilution + immunogen at 0.1 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 57 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Detection limit for this antibody is ~0.1ng/ml as a capture antibody.

References for Anti-LAD1 antibody (ab76610)

ab76610 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"