Recombinant fragment Predicted to work with:
Recombinant fragment: SESVKSRGLP CTELFVAPVG VASKRHLFEK ELAGQSRAEP ASSRKENLRL SGVVTSRLNL WISRTQESGD QDPQEAQKAS SATERTQWGQ KSDSSLDAEV , corresponding to amino acids 418-518 of Human LAD1 (NP_005549) with a tag of 26 kDa. Total MW of immunogen is 37 kDa.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 57 kDa). This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Use at an assay dependent dilution. Detection limit for this antibody is ~0.1ng/ml if used as a capture antibody.
FunctionAnchoring filament protein which is a component of the basement membrane zone.