Anti-LAIR1 antibody [NKTA255], prediluted (Phycoerythrin) (ab27755)

Overview

  • Product name
    Anti-LAIR1 antibody [NKTA255], prediluted (Phycoerythrin)
    See all LAIR1 primary antibodies
  • Description
    Mouse monoclonal [NKTA255] to LAIR1, prediluted (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    ab27755 recognises human leukocyte-associated Ig-like receptor-1 (LAIR-1).
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    NK cells lines B12.100 and AM.25 (Human)

  • General notes


    ab27755 is reported to inhibit NK cell triggering via CD16 molecules.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    ab27755 is reported to inhibit NK cell triggering via CD16 molecules.
  • Clonality
    Monoclonal
  • Clone number
    NKTA255
  • Myeloma
    P3U1
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

Use 10µl of neat antibody for 106 cells in 100µl.

Target

  • Function
    Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, downregulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.
  • Tissue specificity
    Expressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, T-cells, B-cells, monocytes, and dendritic cells. Highly expressed in naive T-cells and B-cells but no expression on germinal center B-cells. Abnormally low expression in naive B-cells from HIV-1 infected patients. Very low expression in NK cells from a patient with chronic active Epstein-Barr virus infection.
  • Sequence similarities
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Developmental stage
    Complete loss of expression when naive B-cells proliferates and differentiates into Ig-producing plasma cells under in vitro stimulation.
  • Domain
    ITIM (immunoreceptor tyrosine-based inhibitor motif) motif is a cytoplasmic motif present in 2 copies in the intracellular part of LAIR1. When phosphorylated, ITIM motif can bind the SH2 domain of several SH2-containing phosphatases, leading to down-regulation of cell activation.
  • Post-translational
    modifications
    Phosphorylation at Tyr-251 and Tyr-281 activates it. May be phosphorylated by LCK.
    N-glycosylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5133400O11Rik antibody
    • CD305 antibody
    • D7Bwg0421e antibody
    • hLAIR1 antibody
    • LAIR 1 antibody
    • LAIR-1 antibody
    • LAIR1 antibody
    • LAIR1 protein antibody
    • LAIR1_HUMAN antibody
    • Leukocyte associated Ig like receptor 1 antibody
    • Leukocyte associated immunoglobulin like receptor 1 antibody
    • Leukocyte-associated immunoglobulin-like receptor 1 antibody
    see all

Images

References

This product has been referenced in:
  • Poggi A  et al. p40, a novel surface molecule involved in the regulation of the non-major histocompatibility complex-restricted cytolytic activity in humans. Eur J Immunol 25:369-76 (1995). Read more (PubMed: 7875198) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab27755.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up