Overview

  • Product name
  • Description
    Mouse monoclonal to LAMB3
  • Specificity
    Antibody is generated against the beta subunit (Laminin subunit beta-3, Q13751).
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human LAMB3 aa 1064-1172.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab55415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 130 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Tissue specificity
    Found in the basement membranes (major component).
  • Involvement in disease
    Defects in LAMB3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB) [MIM:226700]; also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic.
    Defects in LAMB3 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • Sequence similarities
    Contains 6 laminin EGF-like domains.
    Contains 1 laminin N-terminal domain.
  • Domain
    The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domain VI is globular.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BM600 125KDA antibody
    • Epiligrin subunit bata antibody
    • FLJ99565 antibody
    • Kalinin B1 chain antibody
    • Kalinin subunit beta antibody
    • LAM5 antibody
    • LAMB3 antibody
    • LAMB3_HUMAN antibody
    • Laminin B1k chain antibody
    • Laminin subunit beta-3 antibody
    • Laminin-5 subunit beta antibody
    • LAMNB1 antibody
    • Nicein subunit beta antibody
    see all

Anti-LAMB3 antibody images



  • Predicted band size : 130 kDa
    Laminin 5 antibody (ab55415) at 1ug/lane + A-431 cell lysate at 25ug/lane.
  • ab55415 at 10 ug/ml staining LAMB3 in human A-431 cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-LAMB3 antibody (ab55415)

This product has been referenced in:
  • Laudanski P  et al. Profiling of selected angiogenesis-related genes in proliferative eutopic endometrium of women with endometriosis. Eur J Obstet Gynecol Reprod Biol N/A:N/A (2013). WB ; Human . Read more (PubMed: 24188612) »
  • Wittig-Blaich SM  et al. Matrix-Dependent Regulation of AKT in Hepsin-Overexpressing PC3 Prostate Cancer Cells. Neoplasia 13:579-89 (2011). WB ; Human . Read more (PubMed: 21750652) »

See all 2 Publications for this product

Product Wall

The immunogen of the antibody is LAMB3 (NP_000219, 1064 a.a. ~ 1172 a.a) partial recombinant protein with GST tag. Immunogen Sequence (without GST): AEGASEQALSAQEGFERIKQKYAELKDRLGQSSMLGEQGARIQSVKTEAE ELFGETMEMMDRMKDMELELLRGSQAIMLRSADLTGLEKRVE...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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