Overview

  • Product nameAnti-LAMB3 antibody
    See all LAMB3 primary antibodies
  • Description
    Mouse monoclonal to LAMB3
  • SpecificityAntibody is generated against the beta subunit (Laminin subunit beta-3, Q13751).
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human LAMB3 aa 1064-1172.

Properties

Applications

Our Abpromise guarantee covers the use of ab55415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 130 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Tissue specificityFound in the basement membranes (major component).
  • Involvement in diseaseDefects in LAMB3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB) [MIM:226700]; also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic.
    Defects in LAMB3 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • Sequence similaritiesContains 6 laminin EGF-like domains.
    Contains 1 laminin N-terminal domain.
  • DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domain VI is globular.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BM600 125KDA antibody
    • Epiligrin subunit bata antibody
    • FLJ99565 antibody
    • Kalinin B1 chain antibody
    • Kalinin subunit beta antibody
    • LAM5 antibody
    • LAMB3 antibody
    • LAMB3_HUMAN antibody
    • Laminin B1k chain antibody
    • Laminin subunit beta-3 antibody
    • Laminin-5 subunit beta antibody
    • LAMNB1 antibody
    • Nicein subunit beta antibody
    see all

Anti-LAMB3 antibody images



  • Predicted band size : 130 kDa
    Laminin 5 antibody (ab55415) at 1ug/lane + A-431 cell lysate at 25ug/lane.
  • ab55415 at 10 ug/ml staining LAMB3 in human A-431 cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-LAMB3 antibody (ab55415)

This product has been referenced in:
  • Laudanski P  et al. Profiling of selected angiogenesis-related genes in proliferative eutopic endometrium of women with endometriosis. Eur J Obstet Gynecol Reprod Biol N/A:N/A (2013). WB ; Human . Read more (PubMed: 24188612) »
  • Wittig-Blaich SM  et al. Matrix-Dependent Regulation of AKT in Hepsin-Overexpressing PC3 Prostate Cancer Cells. Neoplasia 13:579-89 (2011). WB ; Human . Read more (PubMed: 21750652) »

See all 2 Publications for this product

Product Wall

The immunogen of the antibody is LAMB3 (NP_000219, 1064 a.a. ~ 1172 a.a) partial recombinant protein with GST tag. Immunogen Sequence (without GST): AEGASEQALSAQEGFERIKQKYAELKDRLGQSSMLGEQGARIQSVKTEAE ELFGETMEMMDRMKDMELELLRGSQAIMLRSADLTGLEKRVE...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"