Overview

  • Product name
  • Description
    Rabbit polyclonal to LAMB3
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human LAMB3 aa 644-930.

  • Positive control
    • H1299, HeLa, or HepG2 and cell lines. NIH-3T3 and A431 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab97765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 130 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

ICC/IF 1/100 - 1/1000.

Target

  • Function
    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Tissue specificity
    Found in the basement membranes (major component).
  • Involvement in disease
    Defects in LAMB3 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB) [MIM:226700]; also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic.
    Defects in LAMB3 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • Sequence similarities
    Contains 6 laminin EGF-like domains.
    Contains 1 laminin N-terminal domain.
  • Domain
    The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domain VI is globular.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BM600 125KDA antibody
    • Epiligrin subunit bata antibody
    • FLJ99565 antibody
    • Kalinin B1 chain antibody
    • Kalinin subunit beta antibody
    • LAM5 antibody
    • LAMB3 antibody
    • LAMB3_HUMAN antibody
    • Laminin B1k chain antibody
    • Laminin subunit beta-3 antibody
    • Laminin-5 subunit beta antibody
    • LAMNB1 antibody
    • Nicein subunit beta antibody
    see all

Images

  • Anti-LAMB3 antibody (ab97765) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 130 kDa
    7.5% SDS PAGE
  • Anti-LAMB3 antibody (ab97765) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size : 130 kDa
    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa, using ab97765 at 1:200.

  • Immunohistochemical analysis of paraffin-embedded human lung cancer, using ab97765 to detect LAMB3 at 1:250.

References

ab97765 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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