Overview

  • Product nameAnti-Lambda Light chain antibody [EPR5367-62]
    See all Lambda Light chain primary antibodies
  • Description
    Rabbit monoclonal [EPR5367-62] to Lambda Light chain
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Lambda Light chain. Purified Human IgA.

  • Positive control
    • WB: Human tonsil, plasma, thymus and spleen lysates. IHC-P: Human tonsil and colon tissues. ICC/IF: Ramos cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberEPR5367-62
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000. Predicted molecular weight: 25 kDa.

For unpurified use at 1/50000 - 1/200000.

ELISA 1/64000.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

ICC/IF 1/250.

For unpurified use at 1/500 - 1/1000.

Target

  • RelevanceAll five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • Bence Jones protein antibody
    • BJP antibody
    • Constant region of lambda light chains antibody
    • Ig lambda chain C regions antibody
    • ig lambda-6 chain C region antibody
    • IGLC 1 antibody
    • IGLC 2 antibody
    • IGLC 3 antibody
    • IGLC antibody
    • IGLC1 antibody
    • IGLC2 antibody
    • IGLC3 antibody
    • IGLC6 antibody
    • IGLV antibody
    • Immunoglobulin lambda constant 1 antibody
    • Immunoglobulin lambda constant regin 1 antibody
    • immunoglobulin lambda gene cluster antibody
    • Immunoglobulin lambda locus antibody
    • Immunoglobulin lambda variable cluster antibody
    • Immunoglobulin: lambda light chain antibody
    • Mcg marker antibody
    • Paraprotein antibody
    see all

Anti-Lambda Light chain antibody [EPR5367-62] images

  • Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/100000 dilution (purified) + Human plasma lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/20000 dilution (purified) + Human thymus tissue lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Lambda Light chain with purified ab124719 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/Immunofluorescence analysis of Ramos cells labelling Lambda Light chain with purified ab124719 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • All lanes : Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/50000 dilution (unpurified)

    Lane 1 : Human tonsil lysate
    Lane 2 : Human plasma lysate
    Lane 3 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size : 25 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Lambda Light chain with unpurified ab124719 at a dilution of 1/250.

References for Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

This product has been referenced in:
  • Zhang L  et al. Hsp70 inhibition induces myeloma cell death via the intracellular accumulation of immunoglobulin and the generation of proteotoxic stress. Cancer Lett 339:49-59 (2013). WB ; Human . Read more (PubMed: 23887058) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"