Anti-Lambda Light chain antibody [GA170] (Phycoerythrin) (ab1198)

Overview

  • Product name
    Anti-Lambda Light chain antibody [GA170] (Phycoerythrin)
    See all Lambda Light chain primary antibodies
  • Description
    Mouse monoclonal [GA170] to Lambda Light chain (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    This antibody reacts with lambda light chains of human immunoglobulins.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    PBS with 0.5% BSA and 0.1% sodium azide
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    GA170
  • Myeloma
    unknown
  • Isotype
    IgG2a
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1198 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Measurement of lambda light chain in human lysed whole peripheral blood or mononuclear cells separated by density gradient.
    Studies of clonality of B cell lymphomas in tissue.
    Studies of clonality of B cell leukemias in peripheral blood.

    Lambda (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.

    10µl ofLambda (PE) is sufficient for labelling of 1x106 cells.
  • Target

    • Relevance
      All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
    • Cellular localization
      Cytoplasmic
    • Database links
    • Alternative names
      • Bence Jones protein antibody
      • BJP antibody
      • Constant region of lambda light chains antibody
      • Ig lambda chain C regions antibody
      • ig lambda-6 chain C region antibody
      • IGLC 1 antibody
      • IGLC 2 antibody
      • IGLC 3 antibody
      • IGLC antibody
      • IGLC1 antibody
      • IGLC2 antibody
      • IGLC3 antibody
      • IGLC6 antibody
      • IGLV antibody
      • Immunoglobulin lambda constant 1 antibody
      • Immunoglobulin lambda constant regin 1 antibody
      • immunoglobulin lambda gene cluster antibody
      • Immunoglobulin lambda locus antibody
      • Immunoglobulin lambda variable cluster antibody
      • Immunoglobulin: lambda light chain antibody
      • Mcg marker antibody
      • Paraprotein antibody
      see all

    References

    ab1198 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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