Anti-Lambda Light chain antibody [HP6054], prediluted (ab17112)

Overview

  • Product nameAnti-Lambda Light chain antibody [HP6054], prediluted
    See all Lambda Light chain primary antibodies
  • Description
    Mouse monoclonal [HP6054] to Lambda Light chain, prediluted
  • Tested applicationsSuitable for: IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Rat
  • Immunogen

    Purified human IgG myeloma proteins covalently linked to polyaminostyrene micro beads.

  • Positive control
    • Tonsil
  • General notes


    This antibody stains B cell follicles in human lymphoid tissues. The mantle zone shows a mosaic pattern of labeling while the germinal centers show a coarse meshwork pattern of staining. Lambda light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas.

Properties

  • FormPrediluted
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: Inert stabilizer
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesThis antibody stains B cell follicles in human lymphoid tissues. The mantle zone shows a mosaic pattern of labeling while the germinal centers show a coarse meshwork pattern of staining. Lambda light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas.
  • ClonalityMonoclonal
  • Clone numberHP6054
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/1.

Acetone fixed. Prediluted and ready to use. An incubation period of 30 minutes at room temperature is recommended.

IHC-P 1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Prediluted and ready to use. An incubation period of 30 minutes at room temperature is recommended. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.

Target

  • RelevanceAll five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • Bence Jones protein antibody
    • BJP antibody
    • Constant region of lambda light chains antibody
    • Ig lambda chain C regions antibody
    • ig lambda-6 chain C region antibody
    • IGLC 1 antibody
    • IGLC 2 antibody
    • IGLC 3 antibody
    • IGLC antibody
    • IGLC1 antibody
    • IGLC2 antibody
    • IGLC3 antibody
    • IGLC6 antibody
    • IGLV antibody
    • Immunoglobulin lambda constant 1 antibody
    • Immunoglobulin lambda constant regin 1 antibody
    • immunoglobulin lambda gene cluster antibody
    • Immunoglobulin lambda locus antibody
    • Immunoglobulin lambda variable cluster antibody
    • Immunoglobulin: lambda light chain antibody
    • Mcg marker antibody
    • Paraprotein antibody
    see all

Anti-Lambda Light chain antibody [HP6054], prediluted images

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil tissue sections, labelling Lambda Light Chain with ab17112.

References for Anti-Lambda Light chain antibody [HP6054], prediluted (ab17112)

ab17112 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"