Anti-Lambda Light chain antibody (HRP) (ab9007)

Overview

  • Product nameAnti-Lambda Light chain antibody (HRP)
    See all Lambda Light chain primary antibodies
  • Description
    Sheep polyclonal to Lambda Light chain (HRP)
  • ConjugationHRP
  • SpecificityThe unconjugated immunoglobulin gives a single arc when tested by IEP against human serum. Identity has been confirmed by double diffusion (Ouchterlony) against human serum and an anti- HumanLambda of known specificity.
  • Tested applicationsSuitable for: IHC-Fr, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The immunogen used was human lambda-chain, purified from disease state urines and shown to be homogeneous by SDS-PAGE.

  • General notesThe following points should be observed when using this product: 1) Dilutions of the conjugate must be made in a suitable buffer (e.g. 0.15M Phosphate buffered saline,pH7.2) 2) It is recommended that dilutions of the conjugate are made in polypropylene or polyethylene containers. Other materials (e.g. polystyrene, some grades of glass) may be detrimental to the conjugate. 3) Working dilutions of the conjugate should be prepared immediately prior to use. 4) Peroxidase conjugates are adversely affected by the presence of sodium azide, the use of which shouldtherefore be avoided. 5) The addition of Tween 20 (0.05 – 0.1%) to the conjugate diluent buffer is recommended to prevent nonspecificbinding in EIA. It may also be advantageous in other assay systems.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.20
    Preservative: 0.05% Proclin
    Constituents: 1% BSA, PBS, 10% Stabilzyme
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityPolyclonal
  • IsotypeIgG
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/25 - 1/50.
ELISA Use at an assay dependent dilution. To give an A450nm = 1.0 using wells coated with human serum at 1/2000 dilution and TMB as a substrate: 1/6400 – 1/12800
WB Use at an assay dependent dilution. See Abreview

Target

  • RelevanceAll five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • Bence Jones protein antibody
    • BJP antibody
    • Constant region of lambda light chains antibody
    • Ig lambda chain C regions antibody
    • ig lambda-6 chain C region antibody
    • IGLC 1 antibody
    • IGLC 2 antibody
    • IGLC 3 antibody
    • IGLC antibody
    • IGLC1 antibody
    • IGLC2 antibody
    • IGLC3 antibody
    • IGLC6 antibody
    • IGLV antibody
    • Immunoglobulin lambda constant 1 antibody
    • Immunoglobulin lambda constant regin 1 antibody
    • immunoglobulin lambda gene cluster antibody
    • Immunoglobulin lambda locus antibody
    • Immunoglobulin lambda variable cluster antibody
    • Immunoglobulin: lambda light chain antibody
    • Mcg marker antibody
    • Paraprotein antibody
    see all

Anti-Lambda Light chain antibody (HRP) images

  • All lanes : Anti-Lambda Light chain antibody (HRP) (ab9007) at 1/500 dilution

    Lane 1 : Reduced samples (Conditioned medium from HEK293 expressing recombinant Lambda light chain).
    Lane 2 : Non-reduced samples (Conditioned medium from HEK293 expressing recombinant Lambda light chain).

    Secondary
    Donkey anti-sheep HRP at 1/5000 dilution

    Observed band size : 25,170 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa (possible non-specific binding).

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Lambda Light chain antibody (HRP) (ab9007)

ab9007 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Recombinant protein (Human antibody expressed in HEK293 cells)
Specification Human antibody expressed in HEK293 cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Type Direct
Username

Abcam user community

Verified customer

Submitted Feb 24 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (Conditioned medium from HEK293 expressing rec)
Loading amount 0.05 µg
Specification Conditioned medium from HEK293 expressing rec
Gel Running Conditions Reduced Denaturing (9%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jan 27 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"