Anti-Lambda Light chain antibody, prediluted (ab74316)


  • Product nameAnti-Lambda Light chain antibody, prediluted
    See all Lambda Light chain primary antibodies
  • Description
    Rabbit polyclonal to Lambda Light chain, prediluted
  • SpecificityWe have data to indicate that this antibody may not cross react with Dog. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Baboon
  • Immunogen

    Lambda light chains purified from human Bence Jones proteins.

  • Positive control
    • Tonsil


  • FormPrediluted
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.5M Tris HCl, stabilizing protein, pH 7.6
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab74316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIHC-P: Prediluted ready for use. Perform heat mediated antigen retrieval before commencing with IHC staining protocol by boiling tissue sections in 10mM citrate buffer pH 6.0 for 10-20 mins, followed by cooling at RT for 20 mins.
  • Target

    • RelevanceAll five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • Bence Jones protein antibody
      • BJP antibody
      • Constant region of lambda light chains antibody
      • Ig lambda chain C regions antibody
      • ig lambda-6 chain C region antibody
      • IGLC 1 antibody
      • IGLC 2 antibody
      • IGLC 3 antibody
      • IGLC antibody
      • IGLC1 antibody
      • IGLC2 antibody
      • IGLC3 antibody
      • IGLC6 antibody
      • IGLV antibody
      • Immunoglobulin lambda constant 1 antibody
      • Immunoglobulin lambda constant regin 1 antibody
      • immunoglobulin lambda gene cluster antibody
      • Immunoglobulin lambda locus antibody
      • Immunoglobulin lambda variable cluster antibody
      • Immunoglobulin: lambda light chain antibody
      • Mcg marker antibody
      • Paraprotein antibody
      see all

    Anti-Lambda Light chain antibody, prediluted images

    • ab74316 staining Lambda Light chain in Rat lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 25°C; antigen retrieval was by heat mediation in EDTA pH9. Samples were incubated with primary antibody for 16 hours at 25°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • Cytoplasmic staining of B cells in formalin-fixed, paraffin-embedded human tonsil using ab74316 with a peroxidase-conjugated secondary antibody and a DAB chromogen.

    References for Anti-Lambda Light chain antibody, prediluted (ab74316)

    ab74316 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH9
    Sample Rat Tissue sections (Lymph node)
    Specification Lymph node
    Permeabilization No
    Fixative Formaldehyde

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    Submitted Jul 03 2013