Anti-Lamin B Receptor antibody (ab122919)

Overview

  • Product nameAnti-Lamin B Receptor antibody
    See all Lamin B Receptor primary antibodies
  • Description
    Rabbit polyclonal to Lamin B Receptor
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Highly purified recombinant fragment, corresponding to amino acids 1-81 of Mouse Lamin B Receptor.

  • Positive control
    • HeLa cells; HeLa cell crude extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab122919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 71 kDa.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionAnchors the lamina and the heterochromatin to the inner nuclear membrane.
  • Involvement in diseaseDefects in LBR are a cause of Pelger-Huet anomaly (PHA) [MIM:169400]. PHA is an autosomal dominant inherited abnormality of neutrophils, characterized by reduced nuclear segmentation and an apparently looser chromatin structure. Heterozygotes show hypolobulated neutrophil nuclei with coarse chromatin. Presumed homozygous individuals have ovoid neutrophil nuclei, as well as varying degrees of developmental delay, epilepsy, and skeletal abnormalities.
    Defects in LBR are the cause of hydrops-ectopic calcification-moth-eaten skeletal dysplasia (HEM) [MIM:215140]; also known as Greenberg skeletal dysplasia. HEM is a rare autosomal recessive chondrodystrophy characterized by early in utero lethality and, therefore, considered to be nonviable. Affected fetuses typically present with fetal hydrops, short-limbed dwarfism, and a marked disorganization of chondro-osseous calcification and may present with polydactyly and additional nonskeletal malformations.
    Defects in LBR may be a cause of Reynolds syndrome (REYNS) [MIM:613471]. It is a syndrome specifically associating limited cutaneous systemic sclerosis and primary biliray cirrhosis. It is characterized by liver disease, telangiectasia, abrupt onset of digital paleness or cyanosis in response to cold exposure or stress (Raynaud phenomenon), and variable features of scleroderma. The liver disease is characterized by pruritis, jaundice, hepatomegaly, increased serum alkaline phosphatase and positive serum mitochondrial autoantibodies, all consistent with primary biliary cirrhosis.
  • Sequence similaritiesBelongs to the ERG4/ERG24 family.
  • Post-translational
    modifications
    Phosphorylated by CDK1 protein kinase in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures. Phosphorylation of LBR and HP1 proteins may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle.
  • Cellular localizationNucleus inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DHCR 14B antibody
    • DHCR14B antibody
    • Integral nuclear envelope inner membrane protein antibody
    • Lamin-B receptor antibody
    • LBR antibody
    • LBR_HUMAN antibody
    • LMN 2R antibody
    • LMN2R antibody
    • MGC9041 antibody
    • PHA antibody
    • PRO0650 antibody
    see all

Anti-Lamin B Receptor antibody images

  • Identification of Lamin B Receptor in crude extract of HeLa cells by immuno-precipitation using ab122919, followed by Western blotting.
  • Indirect immuno-fluorescence staining of HeLa cells using ab122919.

References for Anti-Lamin B Receptor antibody (ab122919)

ab122919 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Sample Mouse Cell (Neuronal progenitors)
Specification Neuronal progenitors
Permeabilization Yes - PBS 1%BSA 0,1% Saponine
Fixative Paraformaldehyde
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Submitted Oct 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"