Overview

  • Product nameAnti-Lamin B1 antibody
    See all Lamin B1 primary antibodies
  • Description
    Rabbit polyclonal to Lamin B1
  • Tested applicationsSuitable for: IHC-Fr, WB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Lamin B1 aa 570-586 (C terminal).
    Sequence:

    FHQQGTPRASNRSCAIM


    Database link: P20700

  • Positive control
    • Mammary cancer sections; rat brain tissue lysis

Properties

Applications

Our Abpromise guarantee covers the use of ab65986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 0.5 - 1 µg/ml. Formalin or acetone fixed tissues.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 198 kDa).
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use a concentration of 0.5 - 1 µg/ml. Acetone fixed.

Target

  • FunctionLamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
  • Involvement in diseaseDefects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications
    B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
  • Cellular localizationNucleus inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADLD antibody
    • lamin B1 antibody
    • Lamin-B1 antibody
    • LMN antibody
    • LMN2 antibody
    • LMNB antibody
    • Lmnb1 antibody
    • LMNB1_HUMAN antibody
    • MGC111419 antibody
    • OTTHUMP00000159218 antibody
    see all

Anti-Lamin B1 antibody images

  • Immunohistochemical analysis of paraffin-embedded mammary cancer tissue staining Lamin B1 in cytoplasm using ab65986 at 1µg/ml. DAB chromogenic reaction.

  • Anti-Lamin B1 antibody (ab65986) at 1 µg/ml + rat brain tissue lysate

    Predicted band size : 198 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References for Anti-Lamin B1 antibody (ab65986)

This product has been referenced in:
  • Yao Y  et al. MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia. Stem Cell Reports 7:249-62 (2016). WB ; Human . Read more (PubMed: 27509135) »
  • Cordero JB  et al. Oncogenic Ras Diverts a Host TNF Tumor Suppressor Activity into Tumor Promoter. Dev Cell 18:999-1011 (2010). Read more (PubMed: 20627081) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"