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Products:Cell Biology >> Apoptosis >> Nucleus >> Lamins
Overview
Product name
Anti-Lamin B1 antibody - Nuclear Envelope Marker
See all Lamin B1 products (12) ...
Description
Rabbit polyclonal to Lamin B1 - Nuclear Envelope Marker
Specificity
Lamins do not appear to be universally distributed among different cell and tissue types. ab16048 has been shown to react with HeLa cells/lysates in Western blot and ICC. Other cell/tissue types have not been tested.
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Xenopus laevis, Indian Muntjac
Predicted to work with
Chicken, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Mouse Lamin B1.
(Peptide available as ab16375.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
Lamin B1 and Lamin B antibodies are extremely useful as nuclear loading controls for use with nuclear extracts. When using Lamin B1 antibodies as nuclear loading controls, be aware that in apoptotic cells Lamin B1 is cleaved (Kottke TJ et al.). Lamin B1 will also be removed from a nuclear prep if the nuclear membranes are spun out. This antibody was designed to be a nuclear loading control however it has not yet been tested in appropriate lysates.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class V >> Lamins
Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Nuclear Envelope
Cell Biology >> Apoptosis >> Nucleus >> Lamins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: Use a concentration of 0.1 µg/mlDetects a band of approximately 68 kDa (predicted molecular weight: 66 kDa).
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Function
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Involvement in disease
Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
Sequence similarities
Belongs to the intermediate filament family.
Post-translational
modifications
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Cellular localization
Nucleus inner membrane.
Target information above from: UniProt accessionP20700
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADLD antibody
- lamin B1 antibody
- Lamin-B1 antibody
see all
Database links
- Entrez Gene: 396223 Chicken
- Entrez Gene: 4001 Human
- Entrez Gene: 16906 Mouse
- Entrez Gene: 116685 Rat
- Entrez Gene: 379745 Xenopus laevis
- Entrez Gene: 195816 Zebrafish
- Omim: 150340 Human
- SwissProt: P14731 Chicken
see all
Anti-Lamin B1 antibody - Nuclear Envelope Marker images:
Western blot
All lanes : Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) at 1/1000 dilution
Lane 1 : Hela whole cell lysate
Lane 2 : Hela whole cell lysate with Lamin B1 peptide (ab16375) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Alexa fluor goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 66 kDa
Observed band size : 68-70 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
ICC/IF image of ab16048 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16048, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Immunocytochemistry - Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
Human and mouse cells stained with ab16048 (1/500). The cells were fixed in 100% methanol for 6 minutes at -20°C, then washed once in PBS.
A: HeLa cells + ab16048 (green)
B: HeLa cells counterstained with DAPI (blue)
C: 3T3 cells + ab16048 (green)
D: 3T3 cells counterstained with DAPI (blue)
Image courtesy of Marilena Ciciarello and Patrizia Lavia, University 'La Sapienza' CNR, Italy
Immunocytochemistry - Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
Human and mouse cells stained with ab16048 (1/500). The cells were fixed and permeabilized in 4% formaldehyde, 0.2% Tritonm X100 for 10 minutes at room temperature, then washed 3x in PBS.
A: HeLa cells + ab16048 (green)
B: HeLa cells counterstained with DAPI (blue)
C: 3T3 cells + ab16048 (green)
D: 3T3 cells counterstained with DAPI (blue)
Image courtesy of Marilena Ciciarello and Patrizia Lavia, University 'La Sapienza' CNR, Italy
Western blot - Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048) at 1/1000 dilution + Pancreatic cell line - whole cell lysate at 20 µg
Secondary
HRP conjugated goat anti-rabbit antibody at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 66 kDa
Observed band size : 68 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
IHC image of Lamin B1 staining in human liver FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16048, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
References for Anti-Lamin B1 antibody - Nuclear Envelope Marker (ab16048)
This product has been referenced in:
- Murai Jet al. The USP1/UAF1 Complex Promotes Double-Strand Break Repair through Homologous Recombination. Mol Cell Biol 31:2462-9 (2011).Read more (PubMed: 21482670) »
- Dave Net al. Functional cooperation between Snail1 and twist in the regulation of ZEB1 expression during epithelial to mesenchymal transition. J Biol Chem 286:12024-32 (2011). WB.Read more (PubMed: 21317430) »
See all 47 publications for this product
Publishing research using ab16048? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"