Anti-Lamin B1 antibody, prediluted (ab53652)

Overview

  • Product name
    Anti-Lamin B1 antibody, prediluted
    See all Lamin B1 primary antibodies
  • Description
    Rabbit polyclonal to Lamin B1, prediluted
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to C-terminal of human Lamin B1.

  • Positive control
    • Jurkat cells. Breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab53652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1.
IHC-P 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Target

  • Function
    Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
  • Involvement in disease
    Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Post-translational
    modifications
    B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
  • Cellular localization
    Nucleus inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADLD antibody
    • lamin B1 antibody
    • Lamin-B1 antibody
    • LMN antibody
    • LMN2 antibody
    • LMNB antibody
    • Lmnb1 antibody
    • LMNB1_HUMAN antibody
    • MGC111419 antibody
    • OTTHUMP00000159218 antibody
    see all

Images

  • ab53652 (prediluted) staining Lamin B1 in Human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin embedded tissue.
  • Anti-Lamin B1 antibody (ab74393) at 1/25 dilution + lysate prepared from HepG2 cells

    Observed band size: 67 kDa (why is the actual band size different from the predicted?)



    ab74393 used in the image is the undiluted version of ab53652.

References

ab53652 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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