Overview

  • Product nameAnti-Lamin B1 antibody [ZL-5]
    See all Lamin B1 primary antibodies
  • Description
    Mouse monoclonal [ZL-5] to Lamin B1
  • SpecificityThe antibody recognizes the 68 kDa lamin B1 isoform. Reactivity with other lamin isoforms has not been detected.
  • Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Recombinant human Lamin B.

Properties

Applications

Our Abpromise guarantee covers the use of ab20396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/20. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 18798337
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 68 kDa.
IP Use a concentration of 5 µg/ml.
ELISA Use a concentration of 0.2 - 1 µg/ml.

Target

  • FunctionLamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
  • Involvement in diseaseDefects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modifications
    B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
  • Cellular localizationNucleus inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADLD antibody
    • lamin B1 antibody
    • Lamin-B1 antibody
    • LMN antibody
    • LMN2 antibody
    • LMNB antibody
    • Lmnb1 antibody
    • LMNB1_HUMAN antibody
    • MGC111419 antibody
    • OTTHUMP00000159218 antibody
    see all

Anti-Lamin B1 antibody [ZL-5] images

  • Anti-Lamin B1 antibody [ZL-5] (ab20396) at 0.2 µg/ml + HeLa cell lysates
  • Overlay histogram showing HeLa cells stained with ab20396 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20396, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave no signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-Lamin B1 antibody [ZL-5] (ab20396)

This product has been referenced in:
  • Furfaro AL  et al. HO-1 up-regulation: a key point in high-risk neuroblastoma resistance to bortezomib. Biochim Biophys Acta 1842:613-22 (2014). WB . Read more (PubMed: 24380881) »
  • Snodgrass SM  et al. Tgf-ß1 inhibits Cftr biogenesis and prevents functional rescue of ?F508-Cftr in primary differentiated human bronchial epithelial cells. PLoS One 8:e63167 (2013). WB ; Human . Read more (PubMed: 23671668) »

See all 8 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - nuclear (Mouse Gatsrocnemius)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 50 µg
Specification Mouse Gatsrocnemius
Blocking step Seablock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
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Verified customer

Submitted Nov 30 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa)
Total protein in input 1000 µg
Specification HeLa
Immuno-precipitation step Other - Dynabeads protein G
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Submitted Jul 18 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (beta-cells line)
Loading amount 25 µg
Specification beta-cells line
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Verified customer

Submitted May 14 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"