Overview

  • Product name
  • Description
    Rabbit polyclonal to Lamin B2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 277 - 560 of Human Lamin B2.

  • Positive control
    • U87-MG cell lysate; HeLa cells; Human OVCA tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 68 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
  • Involvement in disease
    Defects in LMNB2 are a cause of partial acquired lipodystrophy (APLD) [MIM:608709]. A rare childhood disease characterized by loss of subcutaneous fat from the face and trunk. Fat deposition on the pelvic girdle and lower limbs is normal or excessive. Most frequently, onset between 5 and 15 years of age. Most affected subjects are females and some show no other abnormality, but many develop glomerulonephritis, diabetes mellitus, hyperlipidemia, and complement deficiency. Mental retardation in some cases. APLD is a sporadic disorder of unknown etiology.
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Post-translational
    modifications
    B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
  • Cellular localization
    Nucleus inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LAMB 2 antibody
    • LAMB2 antibody
    • Lamin-B2 antibody
    • LMN 2 antibody
    • LMN B2 antibody
    • LMN2 antibody
    • LMNB 2 antibody
    • LMNB2 antibody
    • LMNB2_HUMAN antibody
    • MGC2721 antibody
    • RGD1563803 antibody
    see all

Images

  • Anti-Lamin B2 antibody (ab155319) at 1/10000 dilution + U87-MG cell lysate at 30 µg

    Predicted band size: 68 kDa



    7.5% SDS PAGE
  • Immunofluorescence of paraformaldehyde-fixed HeLa cells labeling Lamin B2 using ab155319 at 1/500 dilution (green). Bottom image shows costaining with DNA probe.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Ovarian carcinoma tissue labeling Lamin B with ab155319 at 1/250 dilution.

References

ab155319 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab155319.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up