Overview

  • Product nameAnti-Laminin 1+2 antibody
  • Description
    Rabbit polyclonal to Laminin 1+2
  • SpecificityThis antibody is pan-specific and reacts well with all laminin isoforms tested: laminin-1 (alpha-1, beta-1, and gamma-1) and laminin-2 (alpha-2, beta-1, and gamma-1).
  • Tested applicationsSuitable for: IHC-Fr, Inhibition Assay, IHC-P, WB, ICC/IF, IHC-FrFlmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Human
  • Immunogen

    Full length native protein (purified) (Mouse EHS tumour).

  • Positive control
    • Basement membranes (blood vessels of any tissue, tumors and nerve).
  • General notes


    Laminin antibodies are widely used to label blood vessels and basement membranes.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesLaminin antibodies are widely used to label blood vessels and basement membranes.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/500 - 1/2000.
Inhibition Assay Use at an assay dependent concentration. This product functionally inhibits laminin in mouse and rat. Not tested in human tissue.
IHC-P 1/500 - 1/2000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

Immunostaining enhanced by antigen retrieval with pepsin - apply 100ul of 4mg/ml pepsin dissolved in 0.01M HCl for 1 hour at 37C,  especially paraffin tissue.

WB 1/100 - 1/5000.

Use 5-7% of gel for electrophoresis.

ICC/IF 1/50 - 1/200.
IHC-FrFl Use at an assay dependent concentration. PubMed: 25077029

Target

Anti-Laminin 1+2 antibody images

  • All lanes : Anti-Laminin 1+2 antibody (ab7463) at 1 µg/ml

    Lane 1 : EHS
    Lane 2 : Heart


    Observed band size : 200,400 kDa (why is the actual band size different from the predicted?)
  • Ab7463 staining Laminin in rat spinal cord and dorsal root by immunohistochemistry using paraformaldehyde/paraffin embedded tissue. Pepsin antigen retrieval was performed on this tissue sample.
  • ab7463 staining Laminin 1+2 in human MDA-MB-231 by ICC/IF (Immunocytochemistry/ Immunofluorescence). The cells were paraformaldehyde fixed, permeabilized in 0.1% Triton X-100 buffer and blocked in 5% serum for 30 minutes at 25°C. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 37°C. A Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used undiluted, as secondary.

References for Anti-Laminin 1+2 antibody (ab7463)

This product has been referenced in:
  • Phua DC  et al. ZO-1 and ZO-2 are required for extra-embryonic endoderm integrity, primitive ectoderm survival and normal cavitation in embryoid bodies derived from mouse embryonic stem cells. PLoS One 9:e99532 (2014). ICC/IF ; Mouse . Read more (PubMed: 24905925) »
  • Isasi E  et al. Increased blood-brain barrier permeability and alterations in perivascular astrocytes and pericytes induced by intracisternal glutaric acid. Fluids Barriers CNS 11:15 (2014). IHC-FrFl ; Rat . Read more (PubMed: 25077029) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (MDA-MB-231)
Specification MDA-MB-231
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 06 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (normal brain)
Specification normal brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step TNB Blocking Reagent (from another company) as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 28 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Acetone
Permeabilization No
Blocking step TNB Blocking Reagent (from another company) as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 28 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (intestinal mucosa)
Loading amount 70 µg
Specification intestinal mucosa
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Sofia Tedelind

Verified customer

Submitted Feb 11 2008

Thank you for your enquiry. This antibody will reavt with beta 1 and gamma 1 and reacts with mouse. I am not sure that I understand your question about the immunofluorescence though. This antibody has been tested for use in staining of frozen and paraf...

Read More

I was informed that unfortunately, we do not know the protein concentration. For practical purposes, I would recommend using the isotype control at a concentration in the range of 1 mg/ml. I'm sorry I couldn't help you more on this occasion,

Unfortunately, we do not know what chain the antibody is directed against specifically. If you have any more questions, please contact us again.

The following should be helpful: The fixation is routine paraformaldehyde or formalin fixation of tissue prior to paraffin embedding. However, the staining procedure must include an antigen retrieval step pretreating the deparaffinized sections wit...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"