Overview

  • Product name
    Anti-Laminin 2 alpha antibody [4H8-2]
    See all Laminin 2 alpha primary antibodies
  • Description
    Rat monoclonal [4H8-2] to Laminin 2 alpha
  • Host species
    Rat
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, WB, ELISA, ICC, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Heart Laminin 2 (Mouse).

  • Epitope
    The epitope recognized by the antibody resides in the N-terminal portion of the alpha 2 chain of laminin.
  • Positive control
    • Human tongue.
  • General notes
    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab11576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 4 - 8 µg/ml.

This concentration was determined for acetone-fixed frozen sections of human tongue.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 400 kDa.
ELISA Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function
    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Tissue specificity
    Placenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone.
  • Involvement in disease
    Defects in LAMA2 are the cause of merosin-deficient congenital muscular dystrophy type 1A (MDC1A) [MIM:607855]. MDC1A is characterized by difficulty walking, hypotonia, proximal weakness, hyporeflexia, and white matter hypodensity on MRI.
  • Sequence similarities
    Contains 17 laminin EGF-like domains.
    Contains 5 laminin G-like domains.
    Contains 2 laminin IV type A domains.
    Contains 1 laminin N-terminal domain.
  • Domain
    The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI, IV and G are globular.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix > basement membrane. Major component.
  • Information by UniProt
  • Database links
    • Alternative names
      • LAMA 2 antibody
      • LAMA2 antibody
      • LAMA2_HUMAN antibody
      • Laminin alpha 2 (merosin congenital muscular dystrophy) antibody
      • Laminin alpha 2 antibody
      • Laminin alpha 2 chain antibody
      • Laminin alpha 2 subunit antibody
      • Laminin M antibody
      • Laminin M chain antibody
      • Laminin subunit alpha-2 antibody
      • Laminin-12 subunit alpha antibody
      • Laminin-2 subunit alpha antibody
      • Laminin-4 subunit alpha antibody
      • LAMM antibody
      • Merosin heavy chain antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)
      Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)This image is courtesy of an Abreview submitted by Dr Ling Guo

      ab11576 staining C57B6 mouse skeletal muscle cryostate tissue sections by IHC-Fr.  The section was acetone fixed and blocked with 5% BSA for 30 mintes at RT, prior to incubation with the primary antibody (diluted 1/200 in blocking buffer) for 2 hours.  A rhodamine-conjugated goat anti-rat antibody was used as the secondary.

      See Abreview

    References

    This product has been referenced in:
    • Aguilar CA  et al. Multiscale analysis of a regenerative therapy for treatment of volumetric muscle loss injury. Cell Death Discov 4:33 (2018). Read more (PubMed: 29531830) »
    • Mead AF  et al. Fundamental constraints in synchronous muscle limit superfast motor control in vertebrates. Elife 6:N/A (2017). Read more (PubMed: 29165242) »

    See all 21 Publications for this product

    Publishing research using ab11576? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    4 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Frozen sections) abreview for Anti-Laminin 2 alpha antibody [4H8-2]

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Guinea pig Tissue sections (Skeletal muscle)
    Permeabilization
    No
    Specification
    Skeletal muscle
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Acetone
    Read More
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    Abcam user community

    Verified customer

    Submitted Nov 13 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Laminin 2 alpha antibody [4H8-2]

     Good
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer
    Permeabilization
    Yes - pH 9.0 Citrate Buffer Antigen Retrieval
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Feb 07 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-Laminin 2 alpha antibody [4H8-2]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (muscle)
    Permeabilization
    Yes - PBS triton0.5%
    Specification
    muscle
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
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    Abcam user community

    Verified customer

    Submitted Mar 14 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-Laminin 2 alpha antibody [4H8-2]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (C57B6 mouse skeletal muscle cryostate sections)
    Specification
    C57B6 mouse skeletal muscle cryostate sections
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Feb 13 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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