Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)
- Datasheet
- Specific References (14)
- Protocols
Overview
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Product nameAnti-Laminin 2 alpha antibody [4H8-2]
See all Laminin 2 alpha primary antibodies -
DescriptionRat monoclonal [4H8-2] to Laminin 2 alpha
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Tested applicationsSuitable for: IHC-Fr, ICC/IF, WB, ELISA, ICC, IPmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Heart Laminin 2 (Mouse).
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EpitopeThe epitope recognized by the antibody resides in the N-terminal portion of the alpha 2 chain of laminin.
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Positive control
- Human tongue.
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General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 15mM Sodium Azide
Constituents: 0.01M Phosphate, pH 7.4 -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone number4H8-2
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab11576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use a concentration of 4 - 8 µg/ml. This concentration was determined for acetone-fixed frozen sections of human tongue. |
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| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 400 kDa. | |
| ELISA | Use at an assay dependent concentration. | |
| ICC | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. |
Target
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FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
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Tissue specificityPlacenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone.
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Involvement in diseaseDefects in LAMA2 are the cause of merosin-deficient congenital muscular dystrophy type 1A (MDC1A) [MIM:607855]. MDC1A is characterized by difficulty walking, hypotonia, proximal weakness, hyporeflexia, and white matter hypodensity on MRI.
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Sequence similaritiesContains 17 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 2 laminin IV type A domains.
Contains 1 laminin N-terminal domain. -
DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
Domains VI, IV and G are globular. -
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. Major component.
- Information by UniProt
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Database links
- Entrez Gene: 3908 Human
- Entrez Gene: 16773 Mouse
- Omim: 156225 Human
- SwissProt: P24043 Human
- SwissProt: Q60675 Mouse
- Unigene: 200841 Human
- Unigene: 256087 Mouse
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Alternative names
- LAMA 2 antibody
- LAMA2 antibody
- LAMA2_HUMAN antibody
see all
Anti-Laminin 2 alpha antibody [4H8-2] images
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![Immunohistochemistry (Frozen sections) - Laminin 2 alpha antibody [4H8-2] (ab11576)](http://a.abcam.com/ps/datasheet/Images/11/ab11576/ab11576_1.jpg)
Immunohistochemistry (Frozen sections) - Laminin 2 alpha antibody [4H8-2] (ab11576)This image is courtesy of an Abreview submitted by Dr Ling Guoab11576 staining C57B6 mouse skeletal muscle cryostate tissue sections by IHC-Fr. The section was acetone fixed and blocked with 5% BSA for 30 mintes at RT, prior to incubation with the primary antibody (diluted 1/200 in blocking buffer) for 2 hours. A rhodamine-conjugated goat anti-rat antibody was used as the secondary.
Protocols
References for Anti-Laminin 2 alpha antibody [4H8-2] (ab11576)
This product has been referenced in:
- Naito M et al. Dnmt3a Regulates Proliferation of Muscle Satellite Cells via p57Kip2. PLoS Genet 12:e1006167 (2016). IHC-Fr . Read more (PubMed: 27415617) »
- Rodgers JT et al. mTORC1 controls the adaptive transition of quiescent stem cells from G0 to G(Alert). Nature 509:393-6 (2014). Mouse . Read more (PubMed: 24870234) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"