Heart Laminin 2 (Mouse).
Our Abpromise guarantee covers the use of ab11576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-Fr | Use a concentration of 4 - 8 µg/ml. This concentration was determined for acetone-fixed frozen sections of human tongue. |
|
ICC/IF | Use at an assay dependent concentration. | |
WB | Use at an assay dependent concentration. Detects a band of approximately 400 kDa. | |
ELISA | Use at an assay dependent concentration. | |
ICC | Use at an assay dependent concentration. | |
IP | Use at an assay dependent concentration. |
ab11576 staining C57B6 mouse skeletal muscle cryostate tissue sections by IHC-Fr. The section was acetone fixed and blocked with 5% BSA for 30 mintes at RT, prior to incubation with the primary antibody (diluted 1/200 in blocking buffer) for 2 hours. A rhodamine-conjugated goat anti-rat antibody was used as the secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"