Anti-Laminin 2 alpha antibody (ab55409)

Overview

  • Product nameAnti-Laminin 2 alpha antibody
    See all Laminin 2 alpha primary antibodies
  • Description
    Mouse monoclonal to Laminin 2 alpha
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment, corresponding to amino acids 3013-3123 of Human Laminin 2 alpha

Properties

Applications

Our Abpromise guarantee covers the use of ab55409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 5 µg/ml.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Target

  • FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Tissue specificityPlacenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone.
  • Involvement in diseaseDefects in LAMA2 are the cause of merosin-deficient congenital muscular dystrophy type 1A (MDC1A) [MIM:607855]. MDC1A is characterized by difficulty walking, hypotonia, proximal weakness, hyporeflexia, and white matter hypodensity on MRI.
  • Sequence similaritiesContains 17 laminin EGF-like domains.
    Contains 5 laminin G-like domains.
    Contains 2 laminin IV type A domains.
    Contains 1 laminin N-terminal domain.
  • DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI, IV and G are globular.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. Major component.
  • Information by UniProt
  • Database links
  • Alternative names
    • LAMA 2 antibody
    • LAMA2 antibody
    • LAMA2_HUMAN antibody
    • Laminin alpha 2 (merosin congenital muscular dystrophy) antibody
    • Laminin alpha 2 antibody
    • Laminin alpha 2 chain antibody
    • Laminin alpha 2 subunit antibody
    • Laminin M antibody
    • Laminin M chain antibody
    • Laminin subunit alpha-2 antibody
    • Laminin-12 subunit alpha antibody
    • Laminin-2 subunit alpha antibody
    • Laminin-4 subunit alpha antibody
    • LAMM antibody
    • Merosin heavy chain antibody
    see all

Anti-Laminin 2 alpha antibody images

  • ab55409 staining Laminin 2 alpha in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with H2O2 for 10 minutes at 25°C; antigen retrieval was by heat mediation in citrate pH 6 at 100°C for 20 minutes. Samples were incubated with primary antibody (1/500) for 20 minutes at 25°C. An undiluted HRP polymer-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Laminin 2 alpha antibody (ab55409) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human pancreas.
  • Western blot against tagged recombinant protein immunogen using ab55409 Laminin 2 alpha antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa

References for Anti-Laminin 2 alpha antibody (ab55409)

ab55409 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate, pH6, 100C, 20 min
Sample Human Tissue sections (placenta)
Specification placenta
Permeabilization No
Fixative Formaldehyde
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Submitted Sep 12 2014

Application Western blot
Sample Human Tissue lysate - whole (Skeletal Muscle)
Loading amount 20 µg
Specification Skeletal Muscle
Gel Running Conditions Reduced Denaturing (3%-8% Tris-Acetate Gel)
Blocking step Li-Cor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
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Submitted Jan 04 2013


After conversing with my colleagues, I would like to offer a free of charge replacement with ab11576. I agree that the datasheet for ab55409 is slightly misleading in listing WB and human under application and species tested, even though it has on...

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Since we do not have any data on endogeneous Laminin 2 alpha in WB, it could be very likely that the bands you are detecting are specific Laminin 2 alpha subunits present in your samples due to postranscriptional or posttranslational modifications...

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As stated on the datasheet for ab55409, the antibody has only been tested and guaranteed to detect the recombinant fragment immunogen in Western Blot. We have no data on the detection of endogenous protein in Western Blot, and it is very likely ba...

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Thank you for contacting Abcam.

It seems our datasheet for ab55409 is incorrect. The laminin 2 alpha protein is a 344 kDa protein (http://www.uniprot.org/uniprot/P24043); thus, when post translational glycosylation is taken into account, the e...

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I apologize for the confusion. The 38kDa is only for the recombinant fragment used as the immunogen. We do not have any data on endogeneous Laminin 2 alpha in WB. The extra 50kDa band could be sample specific subunit of Laminin 2 alpha due to postra...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"