Recombinant
RabMAb

Anti-Laminin 5 alpha 3 antibody [EPR8266] (ab151715)

Overview

  • Product name
    Anti-Laminin 5 alpha 3 antibody [EPR8266]
    See all Laminin 5 alpha 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR8266] to Laminin 5 alpha 3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Laminin 5 alpha 3 (UniProt: Q16787).

  • Positive control
    • HeLa, HepG2, A431, Human skin and SH-SY5Y lysates; HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 367 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Relevance
      Laminins are basement membrane components thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin 5 is an isoform composed of three distinct subunits, alpha 3, beta 3 and gamma 2, which are bound to each other in a cross-shaped molecule by disulphide bonds. It is a complex glycoprotein thought to be involved in cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes. It is also involved in signal transduction via tyrosine phosphorylation of pp125-FAK and p80, and differentiation of keratinocytes. The laminin alpha 3 subunit is also thought to be a component of laminin 6 and laminin 7.
    • Cellular localization
      Secreted
    • Database links
    • Alternative names
      • E170 antibody
      • Epiligrin 170 kDa subunit antibody
      • Epiligrin subunit alpha antibody
      • Kalinin subunit alpha antibody
      • LAMA3 antibody
      • LAMA3A antibody
      • Laminin 5 alpha3 antibody
      • Laminin A3 antibody
      • laminin alpha 3 antibody
      • laminin alpha3 antibody
      • Laminin subunit alpha-3 antibody
      • Laminin-5 subunit alpha antibody
      • Laminin-6 subunit alpha antibody
      • Laminin-7 subunit alpha antibody
      • laminin5 alpha 3 antibody
      • Laminin5 alpha3 antibody
      • LAMNA antibody
      • LOCS antibody
      • Nicein subunit alpha antibody
      see all

    Images

    • Immunofluorescent analysis of HepG2 cells labeling Laminin 5 alpha 3 with ab151715 at 1/100 dilution.
    • All lanes : Anti-Laminin 5 alpha 3 antibody [EPR8266] (ab151715) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : Human skin lysate
      Lane 5 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 367 kDa

    References

    ab151715 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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