Overview

  • Product name
  • Description
    Rabbit polyclonal to Laminin 5
  • Tested applications
    Suitable for: IP, ICC/IF, IHC-Fr, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Horse, Dog, Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Laminin 5.

  • Positive control
    • keratinocytes

Properties

Applications

Our Abpromise guarantee covers the use of ab14509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 16940506
ICC/IF Use at an assay dependent concentration. PubMed: 16940506
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 165, 140, 105 kDa.
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature.
  • Database links

Images

  • Anti-Laminin 5 antibody (ab14509) at 5 µg/ml + Cell culture supernatant of keratinocytes at 12 µl

    Secondary
    Peroxidase Conjugated Goat Anti-Rabbit Antibody
  • ICC/IF image of ab14509 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14509, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Yang S  et al. Abnormalities in the basement membrane structure promote basal keratinocytes in the epidermis of hypertrophic scars to adopt a proliferative phenotype. Int J Mol Med 37:1263-73 (2016). IHC-P ; Human . Read more (PubMed: 26986690) »
  • Fedorenko IV  et al. Fibronectin induction abrogates the BRAF inhibitor response of BRAF V600E/PTEN-null melanoma cells. Oncogene 35:1225-35 (2016). WB . Read more (PubMed: 26073081) »

See all 27 Publications for this product

Customer reviews and Q&As

Filter by Application

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Application
Western blot
Sample
Sheep Tissue lysate - whole (Sheep skin)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12%)
Loading amount
25 µg
Specification
Sheep skin
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Suzan Valued Customer

Verified customer

Submitted Aug 25 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Heat Mediate Buffer/Enzyme used (Citrate TBE)
Permeabilization
No
Specification
Uterus
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Ms. Devin Columbus

Verified customer

Submitted Sep 12 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HaCaT)
Loading amount
40 µg
Specification
HaCaT
Gel Running Conditions
Reduced Denaturing (7%, 6 V, 60 min.)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Mar 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Breast)
Loading amount
10 µg
Specification
Breast
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Yoon Pyo Choi

Verified customer

Submitted Jun 25 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (grafted human skin)
Specification
grafted human skin
Fixative
Acetone
Blocking step
10% normal goat serum + 0.1% BSA in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Username

Dr. Cynthia Simbulan-Rosenthal

Verified customer

Submitted Jun 28 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (cervical mucosa)
Specification
cervical mucosa
Username

Abcam user community

Verified customer

Submitted Dec 20 2005

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Purified protein
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Type
Direct
Username

Abcam user community

Verified customer

Submitted Nov 22 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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