Anti-Laminin 5 antibody [P3H9-2] (ab78286)


  • Product nameAnti-Laminin 5 antibody [P3H9-2]
    See all Laminin 5 primary antibodies
  • Description
    Mouse monoclonal [P3H9-2] to Laminin 5
  • Tested applicationsSuitable for: IHC-Fr, IP, ELISA, Inhibition Assay, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Laminin 5. Human keratinocytes

  • Positive control
    • IHC-Fr: normal human colon and skin (acetone fixed) frozen sections.



Our Abpromise guarantee covers the use of ab78286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 1 - 5 µg/ml.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.


  • RelevanceLaminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature.
  • Database links

Anti-Laminin 5 antibody [P3H9-2] images

  • ab78286 staining Laminin 5 in Human cultured skin (NHEK, NB1RGB) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% Blocking One® for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in 10% Blocking One® in PBS) for 1 hour at 25°C. An Alexa Fluor® 555-conjugated Rabbit anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References for Anti-Laminin 5 antibody [P3H9-2] (ab78286)

ab78286 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (foreskin section)
Permeabilization No
Specification foreskin section
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative None

Mr. Julien Coutier

Verified customer

Submitted Jun 20 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Blocking One­ as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Human Tissue sections (cultured skin (NHEK, NB1RGB))
Specification cultured skin (NHEK, NB1RGB)
Permeabilization Yes - 0.1% TritonX-100
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Sep 30 2013

La concentration de ab78286 est de 1mg/ml, le volume unitaire est donc de 100µl.

Thank you for contacting us. For ab78286, Anti-Laminin 5 antibody [P3H9-2], Clone P3H9-2 does not specifically bind to either subunit, it should recognize all. The following literature is about the application of this clone: Oncogen(2007)26, ...

Read More