Tissue, cells or virus corresponding to Human Laminin 5. Human keratinocytes
Our Abpromise guarantee covers the use of ab78286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 130 kDa.|
|IP||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|Inhibition Assay||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 5 µg/ml.|
ab78286 staining Laminin 5 in Human cultured skin (NHEK, NB1RGB) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% Blocking One® for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in 10% Blocking One® in PBS) for 1 hour at 25°C. An Alexa Fluor® 555-conjugated Rabbit anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
ab78286 has not yet been referenced specifically in any publications.