Anti-Laminin 5 antibody [P3H9-2] - BSA and Azide free (ab174717)

Overview

  • Product name
    Anti-Laminin 5 antibody [P3H9-2] - BSA and Azide free
    See all Laminin 5 primary antibodies
  • Description
    Mouse monoclonal [P3H9-2] to Laminin 5 - BSA and Azide free
  • Tested applications
    Suitable for: IHC-Fr, IP, ICC, ELISA, Inhibition Assaymore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Laminin 5. Specifically, human keratinocytes.

  • Positive control
    • IHC-Fr: normal human colon and skin frozen sections.
  • General notes

    This antibody clone is manufactured by Abcam.

    This product is the PBS-only buffer version of ab78286.

Properties

Applications

Our Abpromise guarantee covers the use of ab174717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 1 - 5 µg/ml.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.

Target

  • Relevance
    Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature.
  • Database links

Images

  • This IHC data was generated using the same antibody clone in a different buffer formulation (ab78286). 

    IHC image of Laminin 5 staining in a section of frozen normal human skin*, performed on a Leica Bond™ system using the standard protocol F. No antigen retrieval step was performed prior to staining. The section was incubated with ab78286, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

References

ab174717 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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