Anti-Laminin alpha 5 antibody [4C7] (ab17107)


  • Product nameAnti-Laminin alpha 5 antibody [4C7]
    See all Laminin alpha 5 primary antibodies
  • Description
    Mouse monoclonal [4C7] to Laminin alpha 5
  • SpecificityThis antibody was originally thought to recognize laminin alpha 1 or A, but further characterization has confirmed its specificity for the laminin alpha 5 chain [see Tiger, C.F. (1997) J. Biol. Chem. 272: 28590].
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Full length native protein (purified) corresponding to Human Laminin alpha 5.
    Database link: O15230

  • Positive control
    • Skin
  • General notes

    This antibody reacts with the terminal globular domain of the A chain. The staining with this antibody is sensitive to fixation of paraffin tissues and use of proteolytic enzymatic treatment.



Our Abpromise guarantee covers the use of ab17107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/5 - 1/20. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.


  • RelevanceLaminins are a family of heterotrimeric extracellular glycoproteins consisting of alpha, beta, and gamma chains. Laminins are thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin alpha 5 (LAMA5) belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Laminin alpha 5 is the alpha chain in the Laminin 15 complex which is a heterotrimer composed of alpha 5/beta 2/gamma 3 which are bound to each other by disulfide bonds into a cross shaped molecule comprising one long and three short arms with globules at each end.
  • Cellular localizationSecreted; basement membrane.
  • Database links
  • Alternative names
    • KIAA0533 antibody
    • KIAA1907 antibody
    • LAMA5 antibody
    • Laminin alpha 5 chain antibody
    • Laminin subunit alpha 5 antibody
    • Laminin subunit alpha-5 antibody
    • Laminin, alpha 5 antibody
    • Laminin-10 subunit alpha antibody
    • Laminin-11 subunit alpha antibody
    • Laminin-15 subunit alpha antibody
    • RP11-157P1.6 antibody
    see all

Anti-Laminin alpha 5 antibody [4C7] images

  • ab17107 at 1/25 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incubation with the antibody for 16 hours. An HRP conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human renal carcinoma tissue, staining Laminin alpha 5 with ab17107.

References for Anti-Laminin alpha 5 antibody [4C7] (ab17107)

This product has been referenced in:
  • Mackey AL  et al. Sequenced response of extracellular matrix deadhesion and fibrotic regulators after muscle damage is involved in protection against future injury in human skeletal muscle. FASEB J 25:1943-59 (2011). IHC-Fr ; Human . Read more (PubMed: 21368102) »

See 1 Publication for this product

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

The antibody ab5654 is not tested in IHC-P application so Abpromise guarantee will not apply to this product. This antibody was however t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (colon)
Specification colon
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Antigen Unmasking Solution, Citric Acid Based (Vector Lab)
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Jul 03 2007

Thank you for your email. Our source for this antibody has kindly provided me with the following information. In the study by Engvall, 1990, it is stated: "4C7, binds to the globular domain at the end of the long arm (Engvall et al., 1986). This domain...

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This is the only information available to us regarding the immunogen and epitope. Further details can be found in the following two publications: Engvall E et al. Distribution and isolation of four laminin variants; tissue restricted distribution ...

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