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Western blot - Laminin alpha 5 antibody (ab55413)
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Product Name
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Laminin alpha 5 antibody
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See all Laminin alpha 5 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to Laminin alpha 5
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Immunogen
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Recombinant fragment: MGVSLRDKKV HWVYQLGEAG PAVLSIDEDI GEQFAAVSLD RTLQFGHMSV TVERQMIQET KGDTVAPGAE GLLNLRPDDF VFYVGGYPST FTPPPLLRFP , corresponding to amino acids 1-100 of Human Laminin alpha 5
BLAST 'MGVSLRDKKV HWVYQLGEAG PAVLSIDEDI GEQFAAVSLD RTLQFGHMSV TVERQMIQET KGDTVAPGAE GLLNLRPDDF VFYVGGYPST FTPPPLLRFP ' with  or 
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Reacts with
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RecFrag
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Predicted to react
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Hu
(Due to sequence homology)
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted; basement membrane.
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Research areas
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Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Signal Transduction >> Cytoskeleton / ECM >> Basal Lamina
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Relevance
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Laminins are a family of heterotrimeric extracellular glycoproteins consisting of alpha, beta, and gamma chains. Laminins are thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin alpha 5 (LAMA5) belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Laminin alpha 5 is the alpha chain in the Laminin 15 complex which is a heterotrimer composed of alpha 5/beta 2/gamma 3 which are bound to each other by disulfide bonds into a cross shaped molecule comprising one long and three short arms with globules at each end.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Laminin alpha 5 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Laminin alpha 5 antibody images, references, products related to ab55413 and other tools.
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Laminin alpha 5 antibody images:
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 Western blot - Laminin alpha 5 antibody (ab55413)
Western blot against tagged recombinant protein immunogen using ab55413 Laminin alpha 5 antibody at 1ug/ml. Predicted band size of immunogen is 34 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
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Search PubMed (MEDLINE) for references to Laminin alpha 5
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Laminin alpha 5 antibody - more information
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Laminin alpha 5 antibody - related products:
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