Anti-Laminin alpha 5 antibody (ab55413)

Overview

  • Product nameAnti-Laminin alpha 5 antibody
    See all Laminin alpha 5 primary antibodies
  • Description
    Mouse monoclonal to Laminin alpha 5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment: MGVSLRDKKV HWVYQLGEAG PAVLSIDEDI GEQFAAVSLD RTLQFGHMSV TVERQMIQET KGDTVAPGAE GLLNLRPDDF VFYVGGYPST FTPPPLLRFP , corresponding to amino acids 1-100 of Human Laminin alpha 5

Properties

Applications

Our Abpromise guarantee covers the use of ab55413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceLaminins are a family of heterotrimeric extracellular glycoproteins consisting of alpha, beta, and gamma chains. Laminins are thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin alpha 5 (LAMA5) belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Laminin alpha 5 is the alpha chain in the Laminin 15 complex which is a heterotrimer composed of alpha 5/beta 2/gamma 3 which are bound to each other by disulfide bonds into a cross shaped molecule comprising one long and three short arms with globules at each end.
    • Cellular localizationSecreted; basement membrane.
    • Database links
    • Alternative names
      • KIAA0533 antibody
      • KIAA1907 antibody
      • LAMA5 antibody
      • Laminin alpha 5 chain antibody
      • Laminin subunit alpha 5 antibody
      • Laminin subunit alpha-5 antibody
      • Laminin, alpha 5 antibody
      • Laminin-10 subunit alpha antibody
      • Laminin-11 subunit alpha antibody
      • Laminin-15 subunit alpha antibody
      • RP11-157P1.6 antibody
      see all

    Anti-Laminin alpha 5 antibody images

    • Western blot against tagged recombinant protein immunogen using ab55413 Laminin alpha 5 antibody at 1ug/ml. Predicted band size of immunogen is 34 kDa.

      This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    References for Anti-Laminin alpha 5 antibody (ab55413)

    ab55413 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (lung cancer cell)
    Gel Running Conditions Reduced Denaturing (6%)
    Loading amount 100 µg
    Treatment control siRNA & EGFR siRNA :sc-44340 100nM for 72hrs
    Specification lung cancer cell
    Blocking step Milk as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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    Submitted Jun 29 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"