Overview

  • Product name
  • Description
    Rabbit polyclonal to Laminin
  • Specificity
    In dot blot immunoassay this antibody does not react with Fibronectin, Vitronectin, Collagen IV, or Chondroitin sulfate types A, B, and C.
  • Tested applications
    Suitable for: IHC-FoFr, IHC-Fr, WB, IP, IHC - Wholemount, Dot blot, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Horse, Dog, Human, Pig, Xenopus laevis
    Predicted to work with: Reptile, Mammal, Amphibian
  • Immunogen

    Full length native protein (purified) corresponding to Laminin. The immunogen is laminin isolated from the basement membrane of Englebreth Holm-Swarm (EHS) mouse sarcoma.

  • General notes
    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 17418408
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC - Wholemount 1/300. PubMed: 25419850
Dot blot 1/1000. This concentration was determined using laminin at 50 ng per dot.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 20064216

Target

Anti-Laminin antibody images

  • This image shows formalin fixed paraffin embedded human skin stained for Laminin (proteinase K digestion, anti-laminin 1:200 – 30 minutes RT). The picture was kindly supplied as part of the review submitted by Elizabeth Chlipala.
  • ab11575 staining Laminin (red) in mouse postnatal day 14 testes tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and blocked with 0.3% Triton X-100 + 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. A TRITC-conjugated donkey anti-rabbit IgG (H+L) polyclonal (1/200) was used as the secondary antibody. Green - HSD3V1-FITC.

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  • ab11575 staining Laminin in mouse anterior tibialis skeletal muscle tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was permeabilized with 0.5% Triton X in PBS for 5 minutes. Samples were incubated with primary antibody (1/1000 in PBS) for 30 minutes at 25°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/600) was used as the secondary antibody.

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  • ab11575 at a 1/200 dilution staining mouse placenta tissue sections by Immunohistochemistry (frozen sections). The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 12 hours. Bound antibody was detected using an Alexa Fluor ® 594 conjugated goat anti-rabbit IgG.

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  • ab11575 staining Laminin in mouse oocytes by Immunohistochemistry (PFA fixed, paraffin embedded sections).
    Grafts were fixed overnight in 4% paraformaldehyde, embedded in paraffin, and sectioned in 5 to 8 µm intervals. In brief, sections on slides were de-paraffinized, re-hydrated, antigens unmasked by incubating in target retrieval solution at 95°C for 30 minutes, permeabilized in 0.1% Triton-X100 for 5 minutes, blocked with 10% chicken serum in TBST overnight, and incubated with primary antibody at 1/200, in TBST with 1% serum for 1 hour at room temperature. After washing in TBST, slides were incubated with secondary antibody at 1/1000, for 30 minutes at room temperature. Cover slips were mounted with Prolong Gold Antifade with DAPI.

    Immunofluorescence revealed TRA98+ oocytes and FOXL2+ granulosa cells in ovarian cord-like structures (dashed lines) and Laminin+ basement membrane (red) following five days of intact e12.5 female genital ridge transplantation. Alo
  • ab11575 staining Laminin in Mouse skin neoplasia - papilloma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/200) for 8 hours at 4°C. A biotin-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

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  • ab11575 staining Laminin in Mouse skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized with PBST (Triton X 100 0.025%) and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/250) for 1 hour. An undiluted Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody. The image shows 2 differeent skin sections with Laminin (green) staining in the basal layer of the epidermis. DAPI staining in blue.

    See Abreview

References for Anti-Laminin antibody (ab11575)

This product has been referenced in:
  • Moeendarbary E  et al. The soft mechanical signature of glial scars in the central nervous system. Nat Commun 8:14787 (2017). ICC/IF ; Rat . Read more (PubMed: 28317912) »
  • O'Callaghan J  et al. Therapeutic potential of AAV-mediated MMP-3 secretion from corneal endothelium in treating glaucoma. Hum Mol Genet 26:1230-1246 (2017). ICC/IF ; Human . Read more (PubMed: 28158775) »

See all 152 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Tumour plugs)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: 0.1% Trypsin in 0.1% CaCl2
Permeabilization
No
Specification
Tumour plugs
Blocking step
1% BSA + 10% Goat Serum in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Username

Dr. Victoria Thompson

Verified customer

Submitted Mar 16 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Pepsin was included
Permeabilization
No
Specification
Brain
Fixative
Paraformaldehyde
Username

Herr Dr. Alex Liu

Verified customer

Submitted Jan 20 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Cow Tissue (Lung)
Specification
Lung
Username

James Grey

Verified customer

Submitted Dec 19 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Cow Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing (6% Tris-Acetate Gel)
Loading amount
30 µg
Specification
Lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

James Grey

Verified customer

Submitted Dec 19 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic Heart)
Permeabilization
Yes - Tween-20
Specification
Embryonic Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 11 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 05 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% triton X 100
Specification
Brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10 · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Anterior Tibialis Skeletal muscle)
Permeabilization
Yes - 0.5% Triton X in PBS for 5min
Specification
Anterior Tibialis Skeletal muscle
Fixative
None
Username

Abcam user community

Verified customer

Submitted Mar 07 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Anterior Tibialis Skeletal muscle)
Permeabilization
Yes - 0.5% Triton X in PBS for 5min
Specification
Anterior Tibialis Skeletal muscle
Fixative
None
Username

Abcam user community

Verified customer

Submitted Mar 07 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Vastus lateralis skeletal muscle)
Permeabilization
Yes - 0.5% Triton X in PBS for 5min
Specification
Vastus lateralis skeletal muscle
Fixative
None
Username

Abcam user community

Verified customer

Submitted Mar 07 2016

1-10 of 71 Abreviews or Q&A

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