Overview

  • Product nameAnti-Laminin antibodySee all Laminin primary antibodies ...
  • Description
    Rabbit polyclonal to Laminin
  • SpecificityIn dot blot immunoassay this antibody does not react with Fibronectin, Vitronectin, Collagen IV, or Chondroitin sulfate types A, B, and C.
  • Tested applicationsIHC-FoFr, IHC-Fr, WB, IP, Dot Blot, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Horse, Dog, Human, Pig, Xenopus laevis
    Predicted to work with: Reptiles, all Mammals, Amphibians
  • Immunogen

    Protein purified from the basement membrane of Englebreth Holm-Swarm (EHS) sarcoma (Mouse).

  • General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent dilution. PubMed: 17418408
IHC-Fr Use at an assay dependent dilution.
WB Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
Dot Blot 1/1000. This concentration was determined using laminin at 50 ng per dot.
IHC-P 1/25.
ICC/IF Use at an assay dependent dilution. PubMed: 20064216

Target

Anti-Laminin antibody images

  • This image shows formalin fixed paraffin embedded human skin stained for Laminin (proteinase K digestion, anti-laminin 1:200 – 30 minutes RT). The picture was kindly supplied as part of the review submitted by Elizabeth Chlipala.
  • ab11575 at a 1/200 dilution staining mouse placenta tissue sections by Immunohistochemistry (frozen sections). The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 12 hours. Bound antibody was detected using an Alexa Fluor ® 594 conjugated goat anti-rabbit IgG.

    See Abreview

  • ab11575 staining Laminin in mouse oocytes by Immunohistochemistry (PFA fixed, paraffin embedded sections).
    Grafts were fixed overnight in 4% paraformaldehyde, embedded in paraffin, and sectioned in 5 to 8 µm intervals. In brief, sections on slides were de-paraffinized, re-hydrated, antigens unmasked by incubating in target retrieval solution at 95°C for 30 minutes, permeabilized in 0.1% Triton-X100 for 5 minutes, blocked with 10% chicken serum in TBST overnight, and incubated with primary antibody at 1/200, in TBST with 1% serum for 1 hour at room temperature. After washing in TBST, slides were incubated with secondary antibody at 1/1000, for 30 minutes at room temperature. Cover slips were mounted with Prolong Gold Antifade with DAPI.

    Immunofluorescence revealed TRA98+ oocytes and FOXL2+ granulosa cells in ovarian cord-like structures (dashed lines) and Laminin+ basement membrane (red) following five days of intact e12.5 female genital ridge transplantation. Alo
  • ab11575 staining Laminin in Mouse skin neoplasia - papilloma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/200) for 8 hours at 4°C. A biotin-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

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  • ab11575 staining Laminin (red) in mouse postnatal day 14 testes tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and blocked with 0.3% Triton X-100 + 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. A TRITC-conjugated donkey anti-rabbit IgG (H+L) polyclonal (1/200) was used as the secondary antibody. Green - HSD3V1-FITC.

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  • ab11575 staining Laminin in Mouse skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized with PBST (Triton X 100 0.025%) and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/250) for 1 hour. An undiluted Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody. The image shows 2 differeent skin sections with Laminin (green) staining in the basal layer of the epidermis. DAPI staining in blue.

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  • ab11575 staining Laminin in decellularized lung extracellular matrix by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde and blocked with 2% serum for 2 hours at 25°C. Samples were then incubated with ab11575 at a 1/100 dilution for 20 minutes at 4°C. The secondary used was an FITC conjugated goat anti-rabbit IgG used at a 1/100 dilution.

    See Abreview

References for Anti-Laminin antibody (ab11575)

This product has been referenced in:
  • Matthaei M  et al. Endothelial cell microRNA expression in human late-onset Fuchs' dystrophy. Invest Ophthalmol Vis Sci 55:216-25 (2014). Human . Read more (PubMed: 24334445) »
  • Conway MJ  et al. Mosquito saliva serine protease enhances dissemination of dengue virus into the mammalian host. J Virol 88:164-75 (2014). Read more (PubMed: 24131723) »

See all 39 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Tissue sections (Postnatal day 14 testes)
Specification Postnatal day 14 testes
Permeabilization No
Fixative 4%PFA
Username

Dr. Qing Wen

Verified customer

Submitted May 28 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Enzymatic
Sample Mouse Tissue sections (adult testes)
Specification adult testes
Permeabilization No
Fixative 4% PFA
Username

Dr. Qing Wen

Verified customer

Submitted Apr 04 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako serum-free protein block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: proteinaseK
Sample Human Tissue sections (Human skin)
Specification Human skin
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 27 2013

For ab735, anti-p63 (currently unpublished), we do not have any western blot data but we did at one point offer this same clone, 4A4 (referred to on the ab735 datasheet as BC4A4), as a different catalogue item, ab3239. That product was used in the foll...

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Anti-laminin (ab11575) is developed in rabbit using laminin purified from the basement membrane of Englebreth Holm-Swarm (EHS) mouse sarcoma as the immunogen. Since this is a polyclonal antibody it will not have a defined epitope.

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (esophagus)
Specification esophagus
Fixative Formaldehyde
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization No
Username

Dr. Kei Sakamoto

Verified customer

Submitted Nov 20 2012

Vielen Dank für Ihren Anruf.

Hier die Information bezüglich des Immunogens aus unseren internen Daten:

"This source of laminin used to generate the antibody will contain alpha, beta and gamma types of laminin and is pro...

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Thank you for contacting us.

Unfortunately the blocking peptides are not available for these antibodies. You can try negative control i.e. samples without primary antibody or negative cell line or tissue sections as control to compare the resu...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Intestine)
Specification Intestine
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 05 2012

Thank you for contacting us.

The Anti-Laminin antibody (ab11575) is known to detect laminin from Mouse, Rat, Dog, Human, Pig andXenopus laevis. Protein purified from the basement membrane of Englebreth Holm-Swarm (EHS) sarcoma (Mouse) was use...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"