Overview

  • Product nameAnti-Laminin antibody
    See all Laminin primary antibodies
  • Description
    Rabbit polyclonal to Laminin
  • SpecificityIn dot blot immunoassay this antibody does not react with Fibronectin, Vitronectin, Collagen IV, or Chondroitin sulfate types A, B, and C.
  • Tested applicationsSuitable for: IHC-FoFr, IHC-Fr, WB, IP, IHC - Wholemount, Dot blot, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Horse, Dog, Human, Pig, Xenopus laevis
    Predicted to work with: Reptiles, all Mammals, Amphibians
  • Immunogen

    Full length native protein (purified) corresponding to Laminin. The immunogen is laminin isolated from the basement membrane of Englebreth Holm-Swarm (EHS) mouse sarcoma.

  • General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 17418408
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC - Wholemount 1/300. PubMed: 25419850
Dot blot 1/1000. This concentration was determined using laminin at 50 ng per dot.
IHC-P 1/25.
ICC/IF Use at an assay dependent concentration. PubMed: 20064216

Target

Anti-Laminin antibody images

  • This image shows formalin fixed paraffin embedded human skin stained for Laminin (proteinase K digestion, anti-laminin 1:200 – 30 minutes RT). The picture was kindly supplied as part of the review submitted by Elizabeth Chlipala.
  • ab11575 staining Laminin (red) in mouse postnatal day 14 testes tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and blocked with 0.3% Triton X-100 + 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. A TRITC-conjugated donkey anti-rabbit IgG (H+L) polyclonal (1/200) was used as the secondary antibody. Green - HSD3V1-FITC.

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  • ab11575 staining Laminin in mouse anterior tibialis skeletal muscle tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was permeabilized with 0.5% Triton X in PBS for 5 minutes. Samples were incubated with primary antibody (1/1000 in PBS) for 30 minutes at 25°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/600) was used as the secondary antibody.

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  • ab11575 at a 1/200 dilution staining mouse placenta tissue sections by Immunohistochemistry (frozen sections). The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 12 hours. Bound antibody was detected using an Alexa Fluor ® 594 conjugated goat anti-rabbit IgG.

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  • ab11575 staining Laminin in mouse oocytes by Immunohistochemistry (PFA fixed, paraffin embedded sections).
    Grafts were fixed overnight in 4% paraformaldehyde, embedded in paraffin, and sectioned in 5 to 8 µm intervals. In brief, sections on slides were de-paraffinized, re-hydrated, antigens unmasked by incubating in target retrieval solution at 95°C for 30 minutes, permeabilized in 0.1% Triton-X100 for 5 minutes, blocked with 10% chicken serum in TBST overnight, and incubated with primary antibody at 1/200, in TBST with 1% serum for 1 hour at room temperature. After washing in TBST, slides were incubated with secondary antibody at 1/1000, for 30 minutes at room temperature. Cover slips were mounted with Prolong Gold Antifade with DAPI.

    Immunofluorescence revealed TRA98+ oocytes and FOXL2+ granulosa cells in ovarian cord-like structures (dashed lines) and Laminin+ basement membrane (red) following five days of intact e12.5 female genital ridge transplantation. Alo
  • ab11575 staining Laminin in Mouse skin neoplasia - papilloma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/200) for 8 hours at 4°C. A biotin-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

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  • ab11575 staining Laminin in Mouse skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized with PBST (Triton X 100 0.025%) and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/250) for 1 hour. An undiluted Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody. The image shows 2 differeent skin sections with Laminin (green) staining in the basal layer of the epidermis. DAPI staining in blue.

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  • ab11575 staining Laminin in decellularized lung extracellular matrix by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde and blocked with 2% serum for 2 hours at 25°C. Samples were then incubated with ab11575 at a 1/100 dilution for 20 minutes at 4°C. The secondary used was an FITC conjugated goat anti-rabbit IgG used at a 1/100 dilution.

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References for Anti-Laminin antibody (ab11575)

This product has been referenced in:
  • Vanhoutte D  et al. Thrombospondin expression in myofibers stabilizes muscle membranes. Elife 5:N/A (2016). WB ; Mouse . Read more (PubMed: 27669143) »
  • Kristofik N  et al. Impaired von Willebrand factor adhesion and platelet response in thrombospondin-2 knockout mice. Blood 128:1642-50 (2016). Read more (PubMed: 27471233) »

See all 71 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Embryonic Heart)
Permeabilization Yes - Tween-20
Specification Embryonic Heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Oct 11 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 30 µg
Specification Heart
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Oct 05 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step None
Permeabilization Yes - 0.1% triton X 100
Specification Brain
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10 · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted May 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Anterior Tibialis Skeletal muscle)
Permeabilization Yes - 0.5% Triton X in PBS for 5min
Specification Anterior Tibialis Skeletal muscle
Fixative None
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Submitted Mar 07 2016

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Anterior Tibialis Skeletal muscle)
Permeabilization Yes - 0.5% Triton X in PBS for 5min
Specification Anterior Tibialis Skeletal muscle
Fixative None
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Submitted Mar 07 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Vastus lateralis skeletal muscle)
Permeabilization Yes - 0.5% Triton X in PBS for 5min
Specification Vastus lateralis skeletal muscle
Fixative None
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Submitted Mar 07 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (heart)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH=6
Permeabilization No
Specification heart
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative 10% formalin
Username

Ms. Hongyan Zhao

Verified customer

Submitted Oct 27 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Fruit fly (Drosophila melanogaster) Cell (muscle tissue)
Permeabilization Yes - 0.1% Triton
Specification muscle tissue
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Formaldehyde
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Submitted May 19 2015

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Mouse Tissue sections (perfusion fixed brain tissue)
Specification perfusion fixed brain tissue
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
Username

Ms. Eva Borger

Verified customer

Submitted Dec 16 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Sample Pig Tissue sections (Skeletal Muscle)
Specification Skeletal Muscle
Permeabilization No
Fixative Formaldehyde
Username

Dr. Fiona Lewis

Verified customer

Submitted Oct 14 2014

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