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Protein purified from the basement membrane of Englebreth Holm-Swarm (EHS) sarcoma (Mouse).
Our Abpromise guarantee covers the use of ab11575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 17418408|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|Dot Blot||1/1000. This concentration was determined using laminin at 50 ng per dot.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20064216|
|IF||1/300. PubMed: 25419850|
ab11575 staining Laminin (red) in mouse postnatal day 14 testes tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and blocked with 0.3% Triton X-100 + 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. A TRITC-conjugated donkey anti-rabbit IgG (H+L) polyclonal (1/200) was used as the secondary antibody. Green - HSD3V1-FITC.
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