Overview

  • Product name
  • Description
    Rabbit polyclonal to Laminin
  • Specificity
    Laminin fragment P1 100%, collagen type I and IV <0.1%, fibronectin <0.1%
  • Tested applications
    Suitable for: RIA, Sandwich ELISA, ICC/IF, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified laminin P1 fragment from human placenta.

  • Positive control
    • Human skin
  • General notes


    This antibody primarily labels basal membranes (especially s. c. lamina lucida) and may be used to detect disturbances in the basal membrane during tumour invasion.

Properties

Applications

Our Abpromise guarantee covers the use of ab23753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
RIA 1/1000.
Sandwich ELISA Use a concentration of 5 µg/ml. For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab49726 as Detection.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/200 - 1/600.

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase in TBS 15 min at 37°C. There after, non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

ELISA Use at an assay dependent concentration.

Target

  • Function
    Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Tissue specificity
    Broadly expressed in: skin, heart, lung, and the reproductive tracts.
  • Sequence similarities
    Contains 11 laminin EGF-like domains.
    Contains 1 laminin IV type A domain.
    Contains 1 laminin N-terminal domain.
  • Domain
    The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domain IV is globular.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LAMA 1 antibody
    • LAMA antibody
    • LAMA1 antibody
    • LAMB1 antibody
    • Lamc3 antibody
    • LAMC3_HUMAN antibody
    • Laminin A chain antibody
    • Laminin alpha 1 antibody
    • Laminin alpha 1 chain antibody
    • Laminin B1 chain antibody
    • Laminin subunit beta 1 antibody
    • Laminin subunit gamma-3 antibody
    • Laminin-12 subunit gamma antibody
    • Laminin-14 subunit gamma antibody
    • Laminin-15 subunit gamma antibody
    see all

Images

References

ab23753 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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This product has PBS buffer without addition of BSA and preservatives.


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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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