Overview

  • Product nameAnti-Laminin antibody
    See all Laminin primary antibodies
  • Description
    Rabbit polyclonal to Laminin
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Fr, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: all Mammals
  • Immunogen

    Full length native protein isolated from mouse EHS tumor(purified)

  • Positive control
    • This antibody gave a positive signal in MEF1 cell line in IF/ICC.

Properties

Applications

Our Abpromise guarantee covers the use of ab30320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 337 kDa.
ICC/IF 1/100 - 1/2500.
IHC-Fr 1/1000.
IHC-FoFr 1/1000.

Target

  • FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Tissue specificityBroadly expressed in: skin, heart, lung, and the reproductive tracts.
  • Sequence similaritiesContains 11 laminin EGF-like domains.
    Contains 1 laminin IV type A domain.
    Contains 1 laminin N-terminal domain.
  • DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domain IV is globular.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LAMA 1 antibody
    • LAMA antibody
    • LAMA1 antibody
    • LAMB1 antibody
    • Lamc3 antibody
    • LAMC3_HUMAN antibody
    • Laminin A chain antibody
    • Laminin alpha 1 antibody
    • Laminin alpha 1 chain antibody
    • Laminin B1 chain antibody
    • Laminin subunit beta 1 antibody
    • Laminin subunit gamma-3 antibody
    • Laminin-12 subunit gamma antibody
    • Laminin-14 subunit gamma antibody
    • Laminin-15 subunit gamma antibody
    see all

Anti-Laminin antibody images

  • ICC/IF image of ab30320 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30320, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Rat peripheral nerve (low and high magnification; formalin fixed, frozen sections). Immuno-FITC stain (color inverted) with 1:1000 anti-Laminin1 antibody (ab30320).

References for Anti-Laminin antibody (ab30320)

This product has been referenced in:
  • Bruscoli S  et al. Lack of glucocorticoid-induced leucine zipper (GILZ) deregulates B-cell survival and results in B-cell lymphocytosis in mice. Blood 126:1790-801 (2015). WB ; Mouse . Read more (PubMed: 26276664) »
  • Hosaka K  et al. Tumour PDGF-BB expression levels determine dual effects of anti-PDGF drugs on vascular remodelling and metastasis. Nat Commun 4:2129 (2013). Read more (PubMed: 23831851) »

See all 5 Publications for this product

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The antibody is a whole antiserum, without purification. As such, it is a mixture of various proteins, and immunogloblin concentration is not assayed. In general, serum is expected to contain about 10 mg/ml of to...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"