Overview

  • Product nameAnti-Laminin antibody [MEC5]
    See all Laminin primary antibodies
  • Description
    Rat monoclonal [MEC5] to Laminin
  • SpecificityThis antibody reacts with the P1 fragment of laminin.
  • Tested applicationsSuitable for: IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length native protein (purified) corresponding to Rat Laminin. ab2466 was derived from DZB rats that had developed membranous glomerulopathy upon exposure to mercuric chloride

Properties

Applications

Our Abpromise guarantee covers the use of ab2466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/10.

A pepsin pretreatment is required. Especially useful for immunohistochemical detection of laminin in extracellular matrix or basement membranes in freshly frozen tissue sections of rat, mouse or humans.

Target

References for Anti-Laminin antibody [MEC5] (ab2466)

This product has been referenced in:
  • Berthod F  et al. Spontaneous fibroblast-derived pericyte recruitment in a human tissue-engineered angiogenesis model in vitro. J Cell Physiol 227:2130-7 (2012). IHC-Fr ; Human . Read more (PubMed: 21769871) »
  • Aten J  et al. Autoantibodies to the laminin P1 fragment in HgCl2-induced membranous glomerulopathy. Am J Pathol 146:1467-80 (1995). Read more (PubMed: 7778685) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (spinal cord)
Specification spinal cord
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

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Verified customer

Submitted Sep 08 2009

Thank you for your enquiry regarding ab2466. The antigen retrieval method of pepsin treatment is specific for this antibody as it was found that staining was significantly better with this step. This method is classically used for formalin fixed pa...

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Thank you for your enquiry and for your patience. At this time, all the information that we have regarding these two antibodies is located on the online datasheets. Ab6346: MTn-12 (Aufderheide et al. 1988) reacts specifically with mouse tenascin. I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"