The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
Ready to use.
FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Tissue specificityBroadly expressed in: skin, heart, lung, and the reproductive tracts.
Sequence similaritiesContains 11 laminin EGF-like domains. Contains 1 laminin IV type A domain. Contains 1 laminin N-terminal domain.
DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. Domain IV is globular.
ab74164 staining Laminin in human skin by Immunohistochemistry, Formalin-fixed, paraffin-embedded tissue using peroxidase-conjugate and AEC chromogen. Note staining of basement membranes of epidermis and surrounding blood vessels.
References for Anti-Laminin antibody, prediluted (ab74164)