Anti-Laminin beta 1 antibody [LT3] (ab44941)

Overview

  • Product nameAnti-Laminin beta 1 antibody [LT3]
    See all Laminin beta 1 primary antibodies
  • Description
    Rat monoclonal [LT3] to Laminin beta 1
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Fr, IHC (Methanol fixed), IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Hamster, Human, Pig
  • Immunogen

    Murine EHS laminin preparation.

  • Positive control
    • LS174T cells. Normal colon or colon carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab44941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/200. Detects a band of approximately 210 kDa.
IP 1/400.
IHC-Fr 1/400. Use with acetone fixed tissues.
IHC (Methanol fixed) 1/400.
IHC-P Use at an assay dependent dilution.

Target

  • FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
  • Sequence similaritiesContains 13 laminin EGF-like domains.
    Contains 1 laminin IV type B domain.
    Contains 1 laminin N-terminal domain.
  • DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
    Domains VI and IV are globular.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. Major component.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLM antibody
    • Cutis laxa with marfanoid phenotype antibody
    • LAM B1 antibody
    • LAMB 1 antibody
    • LAMB1 antibody
    • LAMB1_HUMAN antibody
    • Laminin B1 antibody
    • Laminin B1 chain antibody
    • Laminin beta 1 chain antibody
    • Laminin beta 1 chain precursor antibody
    • Laminin beta1 antibody
    • Laminin subunit beta 1 antibody
    • Laminin subunit beta-1 antibody
    • Laminin-1 subunit beta antibody
    • Laminin-10 subunit beta antibody
    • Laminin-12 subunit beta antibody
    • Laminin-2 subunit beta antibody
    • Laminin-6 subunit beta antibody
    • Laminin-8 subunit beta antibody
    • LIS5 antibody
    • MGC142015 antibody
    see all

Anti-Laminin beta 1 antibody [LT3] images

  • ICC/IF image of ab44941 staining human prostate cells.  The cells were fixed with 4% paraformaldehyde, permeabilised with 0.8% Triton X-100, and blocked with 20% serum for 1 hour at 24°C.  The primary anitibody was diluted 1/400 in in 20% horse serum in PBS and incubated for 20 minutes at 4°C.  An Alexa Fluor® 488 conjugated donkey anti-rat was used as the secondary antibody.

    The blue staining is the cell nuclei, the green staining shows the laminin beta 1.

    See Abreview

  • ab44941 staining Laminin beta 1 in porcine jejunum tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in methanol/acetone, permeabilized using 0.3% Triton, blocked with 1% normal goat serum for 10 minutes at room temperature and then incubated with ab44941 at a 1/800 dilution for 2 hours. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rat IgG (H+L) used at a 1/200 dilution.

    See Abreview

References for Anti-Laminin beta 1 antibody [LT3] (ab44941)

This product has been referenced in:
  • Little GH  et al. Genome-wide Runx2 occupancy in prostate cancer cells suggests a role in regulating secretion. Nucleic Acids Res 40:3538-47 (2012). WB ; Human . Read more (PubMed: 22187159) »

See 1 Publication for this product

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (melanoma cell line)
Total protein in input 1000 µg
Immuno-precipitation step Protein G
Specification melanoma cell line
Username

Abcam user community

Verified customer

Submitted Nov 18 2015

Thank you for that information.

I have gone ahead and set up a credit for the cost of your original order. Once we recieve the 3 vials of unopened antibody from you the credit will be applied.

When you return the goods, please ensur...

Read More

Thank you for your reply.

Can you please let me know how the unused units of the antibody were stored? If they have been stored at 4C since you received them, I can go ahead and credit the cost of the order back to you and also organize for t...

Read More

Thank you for your reply and for providing that information.

I just had a few more follow up questions:

1 - You received 4 vials of ab44941. How many of the bottles did you test? I ask incase the issue may be limited to a specific b...

Read More

Thank you for contacting Abcam.

I am sorry that you are experiencing some issues with ab44941. The antibody is covered under our Abpromise for six months and is guaranteed to work in IHC-P on Mouse, Hamster, Human & Pig samples . If we ca...

Read More

Thank you for your inquiry.


When conducting IHC or IF, it is generally not advisable to use antibodies which are raised in the same species as the target sample (e.g. a mouse monoclonal antibody against mouse samples). The secondary a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Pig Tissue sections (small intestine (jejunum))
Specification small intestine (jejunum)
Fixative Methanol/Acetone
Permeabilization Yes - 0.3% Triton
Blocking step (agent) for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (prostate)
Specification prostate
Fixative Formaldehyde
Permeabilization Yes - 0.2% Triton-X
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Oct 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Prostate)
Specification Prostate
Fixative Paraformaldehyde
Permeabilization Yes - 0.8% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Jan 07 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"