Anti-Laminin gamma 1 antibody [A5] (Biotin) (ab79670)

Overview

  • Product nameAnti-Laminin gamma 1 antibody [A5] (Biotin)
    See all Laminin gamma 1 primary antibodies
  • Description
    Rat monoclonal [A5] to Laminin gamma 1 (Biotin)
  • ConjugationBiotin
  • Specificityab79670 stains uniformly all human and murine basement membranes.
  • Tested applicationsSuitable for: IP, IHC-Fr, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Murine EHS laminin preparation.

  • Positive control
    • LS174T cells; Normal colon, colon carcinoma and tonsil tissues.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab79670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
IHC-Fr
ICC/IF
IHC-P
  • Application notesICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 2 µg/ml for 30 min at RT. Use Acid-alcohol-fixed tissue.
    Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 min at 37oC.
    IHC-Fr: Use at a concentration of 2 µg/ml for 30 min at RT. Use Acetone-fixed tissue.
    IP: Use at 2µg/mg of lysate (Native and denatured).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
    • Tissue specificityFound in the basement membranes (major component).
    • Sequence similaritiesContains 11 laminin EGF-like domains.
      Contains 1 laminin IV type A domain.
      Contains 1 laminin N-terminal domain.
    • DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
      Domains VI and IV are globular.
    • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • LAMB2 antibody
      • lamc1 antibody
      • LAMC1_HUMAN antibody
      • Laminin B2 chain antibody
      • Laminin subunit gamma-1 antibody
      • Laminin, gamma 1 (formerly LAMB2) antibody
      • Laminin-1 subunit gamma antibody
      • Laminin-10 subunit gamma antibody
      • Laminin-11 subunit gamma antibody
      • Laminin-2 subunit gamma antibody
      • Laminin-3 subunit gamma antibody
      • Laminin-4 subunit gamma antibody
      • Laminin-6 subunit gamma antibody
      • Laminin-7 subunit gamma antibody
      • Laminin-8 subunit gamma antibody
      • Laminin-9 subunit gamma antibody
      • MGC87297 antibody
      • S-LAM gamma antibody
      • S-laminin subunit gamma antibody
      see all

    References for Anti-Laminin gamma 1 antibody [A5] (Biotin) (ab79670)

    ab79670 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"