Anti-Laminin gamma 1 antibody [A5] - BSA and Azide free (ab80580)

Overview

  • Product nameAnti-Laminin gamma 1 antibody [A5] - BSA and Azide free
    See all Laminin gamma 1 primary antibodies
  • Description
    Rat monoclonal [A5] to Laminin gamma 1 - BSA and Azide free
  • SpecificityThis antibody uniformly stains all human and murine basement membranes.
  • Tested applicationsSuitable for: IP, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Murine EHS laminin preparation.

  • Positive control
    • LS174T cells. Normal colon or colon carcinoma.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab80580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
IHC-P
IHC-Fr
ICC/IF
  • Application notesICC/IF: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 2 µg/ml.
    IHC Acetone-fixed Fr: Use at a concentration of 2 µg/ml.
    IP: Use at 2µg/mg of lysate. (Native and denatured)

    Staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000U/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 min at 37°C.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionBinding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
    • Tissue specificityFound in the basement membranes (major component).
    • Sequence similaritiesContains 11 laminin EGF-like domains.
      Contains 1 laminin IV type A domain.
      Contains 1 laminin N-terminal domain.
    • DomainThe alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure.
      Domains VI and IV are globular.
    • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • LAMB2 antibody
      • lamc1 antibody
      • LAMC1_HUMAN antibody
      • Laminin B2 chain antibody
      • Laminin subunit gamma-1 antibody
      • Laminin, gamma 1 (formerly LAMB2) antibody
      • Laminin-1 subunit gamma antibody
      • Laminin-10 subunit gamma antibody
      • Laminin-11 subunit gamma antibody
      • Laminin-2 subunit gamma antibody
      • Laminin-3 subunit gamma antibody
      • Laminin-4 subunit gamma antibody
      • Laminin-6 subunit gamma antibody
      • Laminin-7 subunit gamma antibody
      • Laminin-8 subunit gamma antibody
      • Laminin-9 subunit gamma antibody
      • MGC87297 antibody
      • S-LAM gamma antibody
      • S-laminin subunit gamma antibody
      see all

    Anti-Laminin gamma 1 antibody [A5] - BSA and Azide free images

    • Frozen human tonsil stained with ab80580 at 2µg/ml using peroxidase-conjugate and DAB chromogen. Note basement membrane and cell surface staining of blood vessels.
    • Native IP using LS174T cells:
      IP 2µg/mg lysate.
      WB at 2µg/ml.

    References for Anti-Laminin gamma 1 antibody [A5] - BSA and Azide free (ab80580)

    This product has been referenced in:
    • Ljubimov AV  et al. Human corneal basement membrane heterogeneity: topographical differences in the expression of type IV collagen and laminin isoforms. Lab Invest 72:461-73 (1995). ICC/IF, IHC-P, IHC-Fr, IP ; Human, Mouse . Read more (PubMed: 7723285) »
    • Ljubimov AV  et al. Distribution of individual components of basement membrane in human colon polyps and adenocarcinomas as revealed by monoclonal antibodies. Int J Cancer 50:562-6 (1992). ICC/IF, IHC-P, IHC-Fr, IP ; Human, Mouse . Read more (PubMed: 1371500) »

    See all 3 Publications for this product

    Product Wall

    Thank you for contacting us. We can recommend using antibody ab106783, ab99667, ab99666 etc. Rabbit or goat anti rat IgG also cross reacts with IgG2a so anti rat IgGs can also be used. I hope this information is helpful to you. Please do not hesitate...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"